{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["17(1)"],"submitter":["Wan X"],"pubmed_abstract":["DNA topology is critical for regulating transcription and maintaining cellular homeostasis. Z-DNA is a left-handed DNA helix in regions with high transcriptional activity. Its physiological function remains poorly understood. Here, we demonstrate that oncoprotein MYC induces the formation of Z-DNA by recruiting the chromatin remodeler FACT, independent of RNA Polymerase II activity. FACT facilitates Z-DNA formation by remodeling H2A/H2B dimers within intact nucleosomes. Additionally, the phosphorylation of FACT regulates its liquid-liquid phase separation, promoting its efficient recruitment to chromatin by MYC. Through a genome-wide analysis and characterization of engineered Z-DNA promoters, we found that Z-DNA directly facilitates the loading of RNA Polymerase II, thereby promoting tran"],"journal":["Nature communications"],"pagination":["199"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12780141"],"repository":["biostudies-literature"],"pubmed_title":["MYC drives left-handed Z-DNA formation to shape gene expression."],"pmcid":["PMC12780141"],"pubmed_authors":["Liu Y","Fan H","Li Z","Shao J","Fang D","Zhang Y","Du Y","Wan X","Zeng W","Sun Z","Jia J"],"additional_accession":[]},"is_claimable":false,"name":"MYC drives left-handed Z-DNA formation to shape gene expression.","description":"DNA topology is critical for regulating transcription and maintaining cellular homeostasis. Z-DNA is a left-handed DNA helix in regions with high transcriptional activity. Its physiological function remains poorly understood. Here, we demonstrate that oncoprotein MYC induces the formation of Z-DNA by recruiting the chromatin remodeler FACT, independent of RNA Polymerase II activity. FACT facilitates Z-DNA formation by remodeling H2A/H2B dimers within intact nucleosomes. Additionally, the phosphorylation of FACT regulates its liquid-liquid phase separation, promoting its efficient recruitment to chromatin by MYC. Through a genome-wide analysis and characterization of engineered Z-DNA promoters, we found that Z-DNA directly facilitates the loading of RNA Polymerase II, thereby promoting tran","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-06T12:42:21.937Z","creation":"2026-05-30T03:11:38.679Z"},"accession":"S-EPMC12780141","cross_references":{"pubmed":["41326407"],"doi":["10.1038/s41467-025-66886-3"]}}