{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["3(1)"],"submitter":["Lamont EI"],"pubmed_abstract":["Bacterial gene expression from sites of infection are poorly studied due to low levels of bacterial mRNA present in clinical samples. Here, we develop Transcript-Capture Seq, which uses customizable biotinylated probes generated in-house to enrich bacteria-specific RNA from host samples before Next Generation Sequencing (NGS). This method results in a >200-fold increase in bacterial mRNA reads from mixed samples and allows analysis of the complete bacterial transcriptome from clinical samples. We apply Transcript-Capture to models of tuberculosis (TB) infection as well as sputum samples from TB patients. TB patients exhibit unexplained heterogeneity in disease progression, and the activity of <i>Mycobacterium tuberculosis</i> (Mtb) has been proposed to affect treatment response. By applyin"],"journal":["NAR molecular medicine"],"pagination":["ugaf045"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12783040"],"repository":["biostudies-literature"],"pubmed_title":["Transcript-Capture sequencing enriches mRNA of &lt;i&gt;Mycobacterium tuberculosis&lt;/i&gt; from host samples."],"pmcid":["PMC12783040"],"pubmed_authors":["Ma S","Jones RM","Wilkinson RJ","Lamont EI","Assadi J","Winter J","Sherman DR","Weiner DM","Morrison R","Song T","Barry CE","Yu X","Via LE"],"additional_accession":[]},"is_claimable":false,"name":"Transcript-Capture sequencing enriches mRNA of &lt;i&gt;Mycobacterium tuberculosis&lt;/i&gt; from host samples.","description":"Bacterial gene expression from sites of infection are poorly studied due to low levels of bacterial mRNA present in clinical samples. Here, we develop Transcript-Capture Seq, which uses customizable biotinylated probes generated in-house to enrich bacteria-specific RNA from host samples before Next Generation Sequencing (NGS). This method results in a >200-fold increase in bacterial mRNA reads from mixed samples and allows analysis of the complete bacterial transcriptome from clinical samples. We apply Transcript-Capture to models of tuberculosis (TB) infection as well as sputum samples from TB patients. TB patients exhibit unexplained heterogeneity in disease progression, and the activity of <i>Mycobacterium tuberculosis</i> (Mtb) has been proposed to affect treatment response. By applyin","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-05-30T03:13:05.375Z","creation":"2026-05-30T03:07:25.84Z"},"accession":"S-EPMC12783040","cross_references":{"pubmed":["41522880"],"doi":["10.1093/narmme/ugaf045"]}}