biostudies-literatureUnknown16(1)Othman AANecrophagous flies play a critical role in decomposition and serve as bioindicators of environmental health and pollution. Malaysia's tropical rainforest ecosystems may host many necrophagous fly species, including unique blowflies and their associated bacteria. However, in many forest reserve areas, the diversity of these flies remains poorly studied. This study examines the diversity of necrophagous flies and their associated surface bacteria community, with a particular focus on Phumosia promittens (Walker in J Proc Linn Soc Lond Zool 4:90-96, 1859) in the Bangi Forest Reserve, Universiti Kebangsaan Malaysia. Sampling was conducted across three plots using baited traps, and collected flies were morphologically identified and processed under sterile conditions. Surface bacteria from P. pAMB Express1https://www.ebi.ac.uk/biostudies/studies/S-EPMC12783473biostudies-literatureDiversity of necrophagous flies and microbiome profiling of Phumosia promittens as a rainforest health indicator.PMC12783473Ahmad AOthman AAAdman MAAbd Latif ZEshak ZAboshanab KMMohamed Zain NIfalseDiversity of necrophagous flies and microbiome profiling of Phumosia promittens as a rainforest health indicator.Necrophagous flies play a critical role in decomposition and serve as bioindicators of environmental health and pollution. Malaysia's tropical rainforest ecosystems may host many necrophagous fly species, including unique blowflies and their associated bacteria. However, in many forest reserve areas, the diversity of these flies remains poorly studied. This study examines the diversity of necrophagous flies and their associated surface bacteria community, with a particular focus on Phumosia promittens (Walker in J Proc Linn Soc Lond Zool 4:90-96, 1859) in the Bangi Forest Reserve, Universiti Kebangsaan Malaysia. Sampling was conducted across three plots using baited traps, and collected flies were morphologically identified and processed under sterile conditions. Surface bacteria from P. p2025-01-01T00:00:00Z2025 Dec2026-06-06T12:33:24.661Z2026-05-30T03:11:21.511ZS-EPMC127834734138517310.1186/s13568-025-01994-3