biostudies-literatureZhan QFundamental Research Funds for the Central UniversitiesNational Key R&D Program of Chinathe Jiangsu Province "14th Five-Year Plan" Key Construction DisciplineQinglan Project" of Jiangsu Province in 2025National Natural Science Foundation of China16https://www.ebi.ac.uk/biostudies/studies/S-EPMC12784840biostudies-literatureUnknown15(1)Tarantulas (family Theraphosidae) are ecologically significant invertebrate predators in terrestrial ecosystems, but many species face threats from habitat fragmentation and unsustainable collection for the international pet trade. <i>Brachypelma albiceps</i>, a CITES Appendix II-listed species, lacks comprehensive mitochondrial genome characterization, limiting phylogenetic and evolutionary studies. Here, we report a complete mitochondrial genome sequence for <i>B. albiceps</i> (13,856 bp; GC content 32.84%) and provide detailed annotation. The genome exhibits typical metazoan mitochondrial organization, containing 13 protein-coding genes (PCGs), 22 tRNAs, and 2 rRNAs, with an AT-rich nucleotide composition (67.16%) characteristic of arthropod mitochondria. Comparative analyses of <i>B. aBiologyComplete Mitochondrial Genome Sequencing of &lt;i&gt;Brachypelma albiceps&lt;/i&gt; and Comparative Codon Usage Bias Analysis Across Seven Mygalomorphae Species.PMC127848402022YFC2601200LGZD202507202532100366"Public Security Technology" project (2022)Huang YZhan QChen YHou STang YZhao YXue XZhao XfalseComplete Mitochondrial Genome Sequencing of &lt;i&gt;Brachypelma albiceps&lt;/i&gt; and Comparative Codon Usage Bias Analysis Across Seven Mygalomorphae Species.Tarantulas (family Theraphosidae) are ecologically significant invertebrate predators in terrestrial ecosystems, but many species face threats from habitat fragmentation and unsustainable collection for the international pet trade. <i>Brachypelma albiceps</i>, a CITES Appendix II-listed species, lacks comprehensive mitochondrial genome characterization, limiting phylogenetic and evolutionary studies. Here, we report a complete mitochondrial genome sequence for <i>B. albiceps</i> (13,856 bp; GC content 32.84%) and provide detailed annotation. The genome exhibits typical metazoan mitochondrial organization, containing 13 protein-coding genes (PCGs), 22 tRNAs, and 2 rRNAs, with an AT-rich nucleotide composition (67.16%) characteristic of arthropod mitochondria. Comparative analyses of <i>B. a2025-01-01T00:00:00Z2025 Dec2026-07-05T03:14:07.325Z2026-07-05T03:08:27.007ZS-EPMC127848404151485710.3390/biology15010016