{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Buev VS"],"funding":["Russian Science Foundation"],"pagination":["257"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12785897"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(1)"],"pubmed_abstract":["Spermiogenesis requires extensive molecular and structural remodeling to produce motile sperm. Mutations in the testis-specific RNA methyltransferase NSUN7 are associated with defective fibrous sheath, impaired sperm motility, and male infertility. However, the underlying molecular mechanisms remain poorly understood. Here, we performed proteomic profiling of sorted, elongated, and round spermatids, as well as mature spermatozoa from Nsun7 knockout mice. We showed that NSUN7 is present at all stages of spermiogenesis and is most abundant in round spermatids, which corresponds to the formation of the flagellum and fibrous sheath assembly. Loss of NSUN7 altered the abundance of proteins essential for dynein arm assembly (PIH1D3, CCDC103, CCDC40), intraflagellar transport (IFT122), and fibrou"],"journal":["International journal of molecular sciences"],"pubmed_title":["NSUN7 Regulates Sperm Flagella Formation at All Stages of Spermiogenesis."],"pmcid":["PMC12785897"],"funding_grant_id":["21-64-00006-P"],"pubmed_authors":["Permyakov OA","Zgoda VG","Priymak AV","Novikova SE","Manskikh VN","Dontsova OA","Guseva EA","Grigoryeva OO","Rubtsova MP","Sergiev PV","Averina OA","Buev VS"],"additional_accession":[]},"is_claimable":false,"name":"NSUN7 Regulates Sperm Flagella Formation at All Stages of Spermiogenesis.","description":"Spermiogenesis requires extensive molecular and structural remodeling to produce motile sperm. Mutations in the testis-specific RNA methyltransferase NSUN7 are associated with defective fibrous sheath, impaired sperm motility, and male infertility. However, the underlying molecular mechanisms remain poorly understood. Here, we performed proteomic profiling of sorted, elongated, and round spermatids, as well as mature spermatozoa from Nsun7 knockout mice. We showed that NSUN7 is present at all stages of spermiogenesis and is most abundant in round spermatids, which corresponds to the formation of the flagellum and fibrous sheath assembly. Loss of NSUN7 altered the abundance of proteins essential for dynein arm assembly (PIH1D3, CCDC103, CCDC40), intraflagellar transport (IFT122), and fibrou","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-05-29T03:22:50.913Z","creation":"2026-05-29T03:13:19.146Z"},"accession":"S-EPMC12785897","cross_references":{"pubmed":["41516134"],"doi":["10.3390/ijms27010257"]}}