biostudies-literaturePrangsuwan PNational Research Council of Thailand128https://www.ebi.ac.uk/biostudies/studies/S-EPMC12786085biostudies-literatureUnknown27(1)<i>Streptococcus suis</i> is an important zoonotic pathogen responsible for severe infections in pigs and humans. Its capacity to survive within phagocytic cells is considered a key virulence mechanism that contributes to dissemination and persistence in host tissues. This study employed comparative proteomic profiling to investigate intracellular adaptation of <i>S. suis</i> serotypes 2 (SS2) and 14 (SS14) during infection of human U937 macrophages. Five isolates originating from humans and pigs were analyzed using gel electrophoresis with liquid chromatography-tandem mass spectrometry (GeLC-MS/MS), revealing 118 differentially expressed proteins grouped into 11 functional categories. Translation-related proteins represented the largest group (48%), including upregulated ribosomal subunitInternational journal of molecular sciencesProteomic Analysis of <i>Streptococcus suis</i> During Exposure to Intracellular Condition of Human Macrophage U937 Cells.PMC12786085PK./ 2554-155Yinsai ODuangsonk KPrangsuwan PRoytrakul SfalseProteomic Analysis of <i>Streptococcus suis</i> During Exposure to Intracellular Condition of Human Macrophage U937 Cells.<i>Streptococcus suis</i> is an important zoonotic pathogen responsible for severe infections in pigs and humans. Its capacity to survive within phagocytic cells is considered a key virulence mechanism that contributes to dissemination and persistence in host tissues. This study employed comparative proteomic profiling to investigate intracellular adaptation of <i>S. suis</i> serotypes 2 (SS2) and 14 (SS14) during infection of human U937 macrophages. Five isolates originating from humans and pigs were analyzed using gel electrophoresis with liquid chromatography-tandem mass spectrometry (GeLC-MS/MS), revealing 118 differentially expressed proteins grouped into 11 functional categories. Translation-related proteins represented the largest group (48%), including upregulated ribosomal subunit2025-01-01T00:00:00Z2025 Dec2026-06-09T03:10:13.682Z2026-06-09T03:07:00.401ZS-EPMC127860854151601110.3390/ijms27010128