{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["18(1)"],"submitter":["Dong X"],"pubmed_abstract":["<b>Background/Objectives</b>: Impaired intestinal barrier function is a hallmark of inflammatory bowel disease (IBD). <i>Akkermansia muciniphila</i> and its outer membrane protein Amuc_1100 can enhance this barrier, but the clinical application of Amuc_1100 is limited by the fastidious growth of its native host. This study aimed to overcome this by utilizing the robust probiotic <i>Lacticaseibacillus paracasei</i> L9 for targeted Amuc_1100 delivery. <b>Methods</b>: We engineered <i>Lc. paracasei</i> L9 to express Amuc_1100 via intracellular (pA-L9), secretory (pUA-L9), and surface-display (pUPA-L9) strategies. Their efficacy was assessed in Lipopolysaccharide (LPS)-induced macrophages and a dextran sulfate sodium (DSS)-induced colitis mouse model, evaluating inflammation, barrier integrity"],"journal":["Nutrients"],"pagination":["123"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12787787"],"repository":["biostudies-literature"],"pubmed_title":["Subcellular Localization Dictates Therapeutic Function: Spatially Targeted Delivery of Amuc_1100 by Engineered &lt;i&gt;Lacticaseibacillus paracasei&lt;/i&gt; L9 Enhances Intestinal Barrier in Colitis."],"pmcid":["PMC12787787"],"pubmed_authors":["Ge S","Zhao L","Dong X","Wang R","Zhang H","Zhang M","Miao W","Zhai Z","Hao Y","Ai L","Lin L"],"additional_accession":[]},"is_claimable":false,"name":"Subcellular Localization Dictates Therapeutic Function: Spatially Targeted Delivery of Amuc_1100 by Engineered &lt;i&gt;Lacticaseibacillus paracasei&lt;/i&gt; L9 Enhances Intestinal Barrier in Colitis.","description":"<b>Background/Objectives</b>: Impaired intestinal barrier function is a hallmark of inflammatory bowel disease (IBD). <i>Akkermansia muciniphila</i> and its outer membrane protein Amuc_1100 can enhance this barrier, but the clinical application of Amuc_1100 is limited by the fastidious growth of its native host. This study aimed to overcome this by utilizing the robust probiotic <i>Lacticaseibacillus paracasei</i> L9 for targeted Amuc_1100 delivery. <b>Methods</b>: We engineered <i>Lc. paracasei</i> L9 to express Amuc_1100 via intracellular (pA-L9), secretory (pUA-L9), and surface-display (pUPA-L9) strategies. Their efficacy was assessed in Lipopolysaccharide (LPS)-induced macrophages and a dextran sulfate sodium (DSS)-induced colitis mouse model, evaluating inflammation, barrier integrity","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-09T03:10:56.599Z","creation":"2026-06-09T03:07:27.168Z"},"accession":"S-EPMC12787787","cross_references":{"pubmed":["41515240"],"doi":["10.3390/nu18010123"]}}