{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["75"],"submitter":["Sun YJ"],"pubmed_abstract":["<h4>Introduction</h4>Triple-negative breast cancer (TNBC) has a high mortality rate and limited treatment options. Tetrahydrocurcumin (THC), a major metabolite of curcumin, has potential antitumor activities. However, the antitumor effects and mechanism of THC in TNBC remain elusive.<h4>Objectives</h4>To investigate the mechanism of THC in combating TNBC by targeting TRIP13 to disrupt the interaction of the TRIP13/USP7/c-FLIP complex and mediate c-FLIP ubiquitination both in vitro and in vivo.<h4>Methods</h4>Apoptosis was measured by TUNEL and flow cytometry. Click chemistry-based target fishing, CETSA, DARTS, and SPR were used to identify direct target of THC. Protein interactions was examined using co-immunoprecipitation. The role of USP7 in THC-mediated c-FLIP ubiquitination was evaluat"],"journal":["Journal of advanced research"],"pagination":["591-605"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12789769"],"repository":["biostudies-literature"],"pubmed_title":["Tetrahydrocurcumin targets TRIP13 inhibiting the interaction of TRIP13/USP7/c-FLIP to mediate c-FLIP ubiquitination in triple-negative breast cancer."],"pmcid":["PMC12789769"],"pubmed_authors":["Sun YJ","Sun XH","Qiu F","Zhao CY","Zhang Q","Shang ZB","Cao ZY","Gao XM","Cao SJ","Zhang JC","Zhang QJ","Kang N","Zhang BY"],"additional_accession":[]},"is_claimable":false,"name":"Tetrahydrocurcumin targets TRIP13 inhibiting the interaction of TRIP13/USP7/c-FLIP to mediate c-FLIP ubiquitination in triple-negative breast cancer.","description":"<h4>Introduction</h4>Triple-negative breast cancer (TNBC) has a high mortality rate and limited treatment options. Tetrahydrocurcumin (THC), a major metabolite of curcumin, has potential antitumor activities. However, the antitumor effects and mechanism of THC in TNBC remain elusive.<h4>Objectives</h4>To investigate the mechanism of THC in combating TNBC by targeting TRIP13 to disrupt the interaction of the TRIP13/USP7/c-FLIP complex and mediate c-FLIP ubiquitination both in vitro and in vivo.<h4>Methods</h4>Apoptosis was measured by TUNEL and flow cytometry. Click chemistry-based target fishing, CETSA, DARTS, and SPR were used to identify direct target of THC. Protein interactions was examined using co-immunoprecipitation. The role of USP7 in THC-mediated c-FLIP ubiquitination was evaluat","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-06T13:12:09.176Z","creation":"2026-05-30T03:11:40.744Z"},"accession":"S-EPMC12789769","cross_references":{"pubmed":["39505147"],"doi":["10.1016/j.jare.2024.11.004"]}}