biostudies-literatureUnknown10(1)Delemarre EMBiomarkers are essential in drug development and diagnostics, aiding patient selection and disease monitoring. The lack of age-specific protein references complicates tracking patterns related to chronic disease or treatment. This exploratory, proof-of-concept study explores age-related changes in the serum proteome across the full human lifespan. Using proximity extension assays (Olink), we measured the Immuno-oncology panel in serum from 264 healthy individuals, another panel in a subgroup of 109, all without significant disease at blood draw, aged 0 days to 88 years. Cluster analysis of the Immuno-oncology panel revealed two clusters: cluster 1 included samples from children ≤11 days, cluster 2 encompassing samples with an age range from 2 months till 88 years old. Weighted correlation Advanced biologye00455https://www.ebi.ac.uk/biostudies/studies/S-EPMC12789963biostudies-literatureProteomic Signatures Over Age Reveal Significant Changes From Infancy Till Late Adulthood.PMC12789963van der Klis FRMLeavis HLDrylewicz JNierkens Svan Baarle Dvan Wijk FSmits HMvan Roon JAGWildenbeest JGDelemarre EMvan Houten MAVerhagen LMfalseProteomic Signatures Over Age Reveal Significant Changes From Infancy Till Late Adulthood.Biomarkers are essential in drug development and diagnostics, aiding patient selection and disease monitoring. The lack of age-specific protein references complicates tracking patterns related to chronic disease or treatment. This exploratory, proof-of-concept study explores age-related changes in the serum proteome across the full human lifespan. Using proximity extension assays (Olink), we measured the Immuno-oncology panel in serum from 264 healthy individuals, another panel in a subgroup of 109, all without significant disease at blood draw, aged 0 days to 88 years. Cluster analysis of the Immuno-oncology panel revealed two clusters: cluster 1 included samples from children ≤11 days, cluster 2 encompassing samples with an age range from 2 months till 88 years old. Weighted correlation 2026-01-01T00:00:00Z2026 Jan2026-06-06T11:41:38.649Z2026-05-30T03:07:51.618ZS-EPMC127899634151803810.1002/adbi.202500455