biostudies-literatureUnknown29(1)Kirmse LDahlem Research School and Elsa-Neumann-Stipendium<h4>Purpose</h4>To evaluate different laboratory procedures for determining the etiologic diagnosis of equine recurrent uveitis regarding intraocular infection with Leptospira spp. and to establish a diagnostic guideline.<h4>Material and methods</h4>Eighty horses with a history of ERU were ophthalmologically examined. Serum and aqueous humor were collected. Total protein, albumin level, and MAT against Leptospira spp. were evaluated on serum and aqueous humor. PCR for Leptospira spp., EHV-1 and -4 was performed on aqueous humor. Goldmann-Witmer coefficient (GWC) and C-value (CC) were calculated based on MAT. In 42 cases, an additional ELISA was initiated.<h4>Results</h4>Forty-six female and 34 male horses of different breeds (mean age 10.9 years; range 3 to 31) were included. By MAT 56/80 Veterinary ophthalmologye70132https://www.ebi.ac.uk/biostudies/studies/S-EPMC12790138biostudies-literatureEvaluation of Laboratory Techniques for the Diagnosis of Leptospira-Associated Equine Recurrent Uveitis (ERU) With Focus on the Goldmann-Witmer Coefficient.PMC12790138Kirmse LEule JCThieme KDoherr MGfalseEvaluation of Laboratory Techniques for the Diagnosis of Leptospira-Associated Equine Recurrent Uveitis (ERU) With Focus on the Goldmann-Witmer Coefficient.<h4>Purpose</h4>To evaluate different laboratory procedures for determining the etiologic diagnosis of equine recurrent uveitis regarding intraocular infection with Leptospira spp. and to establish a diagnostic guideline.<h4>Material and methods</h4>Eighty horses with a history of ERU were ophthalmologically examined. Serum and aqueous humor were collected. Total protein, albumin level, and MAT against Leptospira spp. were evaluated on serum and aqueous humor. PCR for Leptospira spp., EHV-1 and -4 was performed on aqueous humor. Goldmann-Witmer coefficient (GWC) and C-value (CC) were calculated based on MAT. In 42 cases, an additional ELISA was initiated.<h4>Results</h4>Forty-six female and 34 male horses of different breeds (mean age 10.9 years; range 3 to 31) were included. By MAT 56/80 2026-01-01T00:00:00Z2026 Jan2026-06-09T05:25:33.712Z2026-06-09T03:07:07.854ZS-EPMC127901384151814710.1111/vop.70132