biostudies-literatureChau THKorea Basic Science InstituteMizutani Foundation for GlycoscienceMacquarie UniversityCancer Institute NSWRoyal Adelaide Hospital Research FundAustralian Research Council101470https://www.ebi.ac.uk/biostudies/studies/S-EPMC12794580biostudies-literatureUnknown25(1)Septic shock, the excessive immune response to pathogen infection, accounts globally for ∼20% of all deaths. Current methods to establish disease severity are unacceptably slow, unspecific, and insensitive, hindering timely and effective treatment. Aiming to establish easy-to-measure glyco-signatures that may identify the most critically unwell patients, we applied comparative glycomics and glycoproteomics to sera longitudinally collected from septic shock survivors (n = 29) and nonsurvivors (n = 8). Glycomics of all 134 serum samples (sampled daily until recovery/death) revealed significant N-glycome dynamics across both patient groups. Unsupervised clustering of the serum N-glycome measured upon intensive care unit (ICU) admission (day 1) indicated survivorship-specific glyco-signatures. We therefore employed machine learning to train a random forest model using the serum N-glycome data. The model accurately classified survivorship outcomes of 35 of 37 patients (accuracy 94.6%) and correctly predicted 29 of 29 survivors (specificity 100%) and six of eight nonsurvivors (sensitivity 75%). Interrogation of the serum N-glycome data revealed that Lewis x (Le^x)-type N-glycans are elevated in nonsurvivors relative to survivors at ICU admission, a finding recapitulated by glycoproteomics. Among the 58 other Le^x-containing serum glycoproteins that were strongly associated with acute phase response and stress pathways, alpha-1-acid-glycoprotein (AGP-1) was identified as a principal carrier of Le^x glycoepitopes with a potential to stratify septic shock survivors from nonsurvivors (AUC 0.90). This study lays a foundation for risk stratification of septic shock patients by uncovering easy-to-assay AGP-1-Le^x glycoforms that identify individuals experiencing poor survival outcomes already upon ICU admission, with the potential to translate to early individualized clinical care at the bedside.Molecular & cellular proteomics : MCPSerum AGP-1-Le<sup>x</sup> Glycoforms Report on Survivorship of Patients with Septic Shock Upon Admission to Intensive Care Unit.PMC127945802500042017152ECF181259FT210100455C52340020224231Traini MKawahara RTorpy DJChernykh AHwang HThaysen-Andersen MMeyer EJFehring JChatterjee SChau THCaulfield LfalseSerum AGP-1-Le<sup>x</sup> Glycoforms Report on Survivorship of Patients with Septic Shock Upon Admission to Intensive Care Unit.Septic shock, the excessive immune response to pathogen infection, accounts globally for ∼20% of all deaths. Current methods to establish disease severity are unacceptably slow, unspecific, and insensitive, hindering timely and effective treatment. Aiming to establish easy-to-measure glyco-signatures that may identify the most critically unwell patients, we applied comparative glycomics and glycoproteomics to sera longitudinally collected from septic shock survivors (n = 29) and nonsurvivors (n = 8). Glycomics of all 134 serum samples (sampled daily until recovery/death) revealed significant N-glycome dynamics across both patient groups. Unsupervised clustering of the serum N-glycome measured upon intensive care unit (ICU) admission (day 1) indicated survivorship-specific glyco-signatures. We therefore employed machine learning to train a random forest model using the serum N-glycome data. The model accurately classified survivorship outcomes of 35 of 37 patients (accuracy 94.6%) and correctly predicted 29 of 29 survivors (specificity 100%) and six of eight nonsurvivors (sensitivity 75%). Interrogation of the serum N-glycome data revealed that Lewis x (Le^x)-type N-glycans are elevated in nonsurvivors relative to survivors at ICU admission, a finding recapitulated by glycoproteomics. Among the 58 other Le^x-containing serum glycoproteins that were strongly associated with acute phase response and stress pathways, alpha-1-acid-glycoprotein (AGP-1) was identified as a principal carrier of Le^x glycoepitopes with a potential to stratify septic shock survivors from nonsurvivors (AUC 0.90). This study lays a foundation for risk stratification of septic shock patients by uncovering easy-to-assay AGP-1-Le^x glycoforms that identify individuals experiencing poor survival outcomes already upon ICU admission, with the potential to translate to early individualized clinical care at the bedside.2026-01-01T00:00:00Z2026 Jan2026-07-04T03:19:20.173Z2026-07-04T03:12:12.649ZS-EPMC127945804126050110.1016/j.mcpro.2025.101470