biostudies-literatureNagai de Lima PMBrown UniversityConselho Nacional de Desenvolvimento Científico e TecnológicoCoordenação de Aperfeiçoamento de Pessoal de Nível Superior425-437https://www.ebi.ac.uk/biostudies/studies/S-EPMC12797236biostudies-literatureUnknown12(1)<i>Candida auris</i> poses a significant healthcare challenge, particularly within immunosuppressed patients. This pathogen can colonize the skin and develop biofilms associated with increased antifungal drug resistance that are difficult to treat with a limited antifungal repertoire. Some adjuvant treatments have been investigated, such as photodynamic therapy (PDT), which employs a photosensitizer (PS) irradiated by light. However, most PSs available suffer from poor biofilm penetration. In this in vitro study, a nanocarrier system was proposed as a possible strategy to facilitate the methylene blue (MB) photosensitizer penetration into biofilm and improve PDT action against <i>C. auris</i>. For this, positively (MB-P) and negatively (MB-N) charged liposomes encapsulating MB were successACS infectious diseasesMethylene Blue-Loaded Liposomal Nanocarriers Enhance the Efficacy of Photodynamic Therapy against &lt;i&gt;Candida auris&lt;/i&gt; Biofilm.PMC12797236310265/2022-388887.700976/2022-0088887.696595/2022-0088887.926029/2023-00407032/2023-1Abbasi ACampos Junqueira JNagai de Lima PMLaMastro VShukla AfalseMethylene Blue-Loaded Liposomal Nanocarriers Enhance the Efficacy of Photodynamic Therapy against &lt;i&gt;Candida auris&lt;/i&gt; Biofilm.<i>Candida auris</i> poses a significant healthcare challenge, particularly within immunosuppressed patients. This pathogen can colonize the skin and develop biofilms associated with increased antifungal drug resistance that are difficult to treat with a limited antifungal repertoire. Some adjuvant treatments have been investigated, such as photodynamic therapy (PDT), which employs a photosensitizer (PS) irradiated by light. However, most PSs available suffer from poor biofilm penetration. In this in vitro study, a nanocarrier system was proposed as a possible strategy to facilitate the methylene blue (MB) photosensitizer penetration into biofilm and improve PDT action against <i>C. auris</i>. For this, positively (MB-P) and negatively (MB-N) charged liposomes encapsulating MB were success2026-01-01T00:00:00Z2026 Jan2026-06-06T15:51:02.796Z2026-06-02T03:09:21.077ZS-EPMC127972364144909910.1021/acsinfecdis.5c00941