{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Osterberg MK"],"funding":["NIAID NIH HHS","Division of Molecular and Cellular Biosciences","Division of Microbiology and Infectious Diseases","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["2808-2826"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12797879"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(11)"],"pubmed_abstract":["The growth of bacterial pathogens is limited by nutritional immunity, where the infected host deploys transition metal scavenging proteins including calprotectin (CP) to starve the bacterium of essential transition metals. Prior work reveals that CP induces a significant Zn- and Fe-starvation response in the Gram-negative opportunistic pathogen <i>Acinetobacter baumannii</i> in liquid culture. Here, we employ a quantitative chemoproteomics platform to pinpoint changes in abundance-corrected cysteine reactivity─and by extension cellular metal occupancy in metalloenzymes─that occur when <i>A. baumannii</i> is challenged with physiological CP in liquid culture relative to an untreated WT control. Changes in protein abundance with CP stress reveal a pronounced Zn-limitation and Fe-starvation r"],"journal":["ACS chemical biology"],"pubmed_title":["Exploring Metalloproteome Remodeling in Calprotectin-Stressed &lt;i&gt;Acinetobacter baumannii&lt;/i&gt; Using Chemoproteomics."],"pmcid":["PMC12797879"],"funding_grant_id":["R01 AI127793","MCA 2122902","R35 GM134964","R35 GM118157","T32 GM131994","R01 AI101171"],"pubmed_authors":["Bak DW","Andreini C","Giedroc DP","Trinidad JC","Akizuki T","Weerapana E","Osterberg MK","Cornish PV","Chazin WJ","Critchlow JM","Skaar EP","Kim M"],"additional_accession":[]},"is_claimable":false,"name":"Exploring Metalloproteome Remodeling in Calprotectin-Stressed &lt;i&gt;Acinetobacter baumannii&lt;/i&gt; Using Chemoproteomics.","description":"The growth of bacterial pathogens is limited by nutritional immunity, where the infected host deploys transition metal scavenging proteins including calprotectin (CP) to starve the bacterium of essential transition metals. Prior work reveals that CP induces a significant Zn- and Fe-starvation response in the Gram-negative opportunistic pathogen <i>Acinetobacter baumannii</i> in liquid culture. Here, we employ a quantitative chemoproteomics platform to pinpoint changes in abundance-corrected cysteine reactivity─and by extension cellular metal occupancy in metalloenzymes─that occur when <i>A. baumannii</i> is challenged with physiological CP in liquid culture relative to an untreated WT control. Changes in protein abundance with CP stress reveal a pronounced Zn-limitation and Fe-starvation r","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-07-15T06:09:52.321Z","creation":"2026-06-30T03:22:40.875Z"},"accession":"S-EPMC12797879","cross_references":{"pubmed":["41151017"],"doi":["10.1021/acschembio.5c00753"]}}