biostudies-literatureUnknownGoldberg JFNHLBI NIH HHS<h4>Background</h4>Circulating microRNAs are promising biomarkers of acute cellular rejection (ACR) and antibody-mediated rejection (AMR) in heart transplantation. The study objective was to assess the characteristics and diagnostic performance of previously identified microRNAs and clinical rejection scores (CRS) in distinct blood samples obtained at the time of an endomyocardial biopsy (EMB).<h4>Methods</h4>In the 5-center, prospective, longitudinal cohort study, GRAfT (Genomic Research Alliance for Transplantation), microRNA sequencing was performed on blood samples. The previously identified microRNAs associated with ACR (n=12) and AMR (n=17) were used to fit a logistic regression model to the current cohort, and the scores were scaled from 0 to 100. Diagnostic performance of ACR and ACirculation. Heart failuree013141https://www.ebi.ac.uk/biostudies/studies/S-EPMC12798699biostudies-literatureIdentification of Heart Transplant Rejection Subtypes With Circulating MicroRNAs.PMC12798699HHSN268201300001CK23 HL143179GRAfT InvestigatorsValantine HABerry GJAgbor-Enoh SShah PRodrigo MEMercado AJang MKong HGoldberg JFNajjar SSHsu STchoukina IMarboe CCShah KBBagchi PfalseIdentification of Heart Transplant Rejection Subtypes With Circulating MicroRNAs.<h4>Background</h4>Circulating microRNAs are promising biomarkers of acute cellular rejection (ACR) and antibody-mediated rejection (AMR) in heart transplantation. The study objective was to assess the characteristics and diagnostic performance of previously identified microRNAs and clinical rejection scores (CRS) in distinct blood samples obtained at the time of an endomyocardial biopsy (EMB).<h4>Methods</h4>In the 5-center, prospective, longitudinal cohort study, GRAfT (Genomic Research Alliance for Transplantation), microRNA sequencing was performed on blood samples. The previously identified microRNAs associated with ACR (n=12) and AMR (n=17) were used to fit a logistic regression model to the current cohort, and the scores were scaled from 0 to 100. Diagnostic performance of ACR and A2026-01-01T00:00:00Z2026 Jan2026-06-06T15:53:31.681Z2026-06-02T03:09:22.232ZS-EPMC127986994152192310.1161/circheartfailure.124.01314110.1161/CIRCHEARTFAILURE.124.013141