biostudies-literatureSun ENational Institutes of Health National Cancer InstituteNational Center for Research ResourcesU.S. Department of Health and Human ServicesNational Institute of Diabetes and Digestive and Kidney DiseasesGeorgia Clinical and Translational Science AllianceVanderbilt Institute for Clinical and Translational ResearchNational Institutes of HealthVanderbilt Cancer Center1185-1194https://www.ebi.ac.uk/biostudies/studies/S-EPMC12799435biostudies-literatureUnknown122(5)<h4>Background</h4>Carnobacterium maltaromaticum and Faecalibacterium prausnitzii induce de novo gut synthesis of vitamin D to inhibit colorectal carcinogenesis in mice. Magnesium (Mg) treatment increases circulating vitamin D, and Mg homeostasis is dependent on TRPM7 genotype.<h4>Objectives</h4>We hypothesize that Mg treatment increases gut C. maltaromaticum and F. prausnitzii, and the effect differs by TRPM7 polymorphism.<h4>Methods</h4>The Personalized Prevention of Colorectal Cancer Trial is a double-blind, precision-based randomized controlled trial with 240 participants randomly assigned to both treatment and TRPM7 genotype. Stool, rectal swabs, and rectal mucosa were collected.<h4>Results</h4>Of 239 participants who completed the trial, 226 with valid microbiome data were analyzed (The American journal of clinical nutritionMagnesium treatment increases gut microbiome synthesizing vitamin D and inhibiting colorectal cancer: results from a double-blind precision-based randomized placebo-controlled trial.PMC12799435UL1 RR024975UL1TR000445P30CA68485VR12960Murff HJAzcarate-Peril MAYu CDai QSun SFan LShrubsole MJSun EZhu XNess RMfalseMagnesium treatment increases gut microbiome synthesizing vitamin D and inhibiting colorectal cancer: results from a double-blind precision-based randomized placebo-controlled trial.<h4>Background</h4>Carnobacterium maltaromaticum and Faecalibacterium prausnitzii induce de novo gut synthesis of vitamin D to inhibit colorectal carcinogenesis in mice. Magnesium (Mg) treatment increases circulating vitamin D, and Mg homeostasis is dependent on TRPM7 genotype.<h4>Objectives</h4>We hypothesize that Mg treatment increases gut C. maltaromaticum and F. prausnitzii, and the effect differs by TRPM7 polymorphism.<h4>Methods</h4>The Personalized Prevention of Colorectal Cancer Trial is a double-blind, precision-based randomized controlled trial with 240 participants randomly assigned to both treatment and TRPM7 genotype. Stool, rectal swabs, and rectal mucosa were collected.<h4>Results</h4>Of 239 participants who completed the trial, 226 with valid microbiome data were analyzed (2025-01-01T00:00:00Z2025 Nov2026-06-06T22:25:26.365Z2026-06-06T03:07:42.174ZS-EPMC127994354094680510.1016/j.ajcnut.2025.09.011