biostudies-literatureUnknown55Johnston PRA paper by Johnston and others published in 2019 provided three <i>Leotiomycetes-</i>wide phylogenies, one based on genomes, one a multigene phylogeny with up to 15 genes, and one based on ITS sequences. The genomic and multigene phylogenies provided a backbone phylogeny for the class that has proven to be robust, providing a phylogenetically stable concept for the large order <i>Helotiales</i>. However, a lack of genomic data for the clades outside of <i>Helotiales</i>, towards the root of the tree, meant that relationships in this part of the tree remained somewhat poorly resolved. The ITS phylogeny, although phylogenetically much less informative, provided a wider taxon coverage, focussing on the type species of genera not otherwise treated for which ITS data was available. This paper tPersoonia527-566https://www.ebi.ac.uk/biostudies/studies/S-EPMC12799595biostudies-literatureAn updated multigene phylogeny of &lt;i&gt;Leotiomycetes&lt;/i&gt;.PMC12799595Johnston PRPark DfalseAn updated multigene phylogeny of &lt;i&gt;Leotiomycetes&lt;/i&gt;.A paper by Johnston and others published in 2019 provided three <i>Leotiomycetes-</i>wide phylogenies, one based on genomes, one a multigene phylogeny with up to 15 genes, and one based on ITS sequences. The genomic and multigene phylogenies provided a backbone phylogeny for the class that has proven to be robust, providing a phylogenetically stable concept for the large order <i>Helotiales</i>. However, a lack of genomic data for the clades outside of <i>Helotiales</i>, towards the root of the tree, meant that relationships in this part of the tree remained somewhat poorly resolved. The ITS phylogeny, although phylogenetically much less informative, provided a wider taxon coverage, focussing on the type species of genera not otherwise treated for which ITS data was available. This paper t2025-01-01T00:00:00Z2025 Dec2026-06-06T15:14:19.424Z2026-06-01T03:10:41.204ZS-EPMC127995954154158010.3114/persoonia.2025.55.13