biostudies-literatureUnknown76(1)Su Q<h4>Background</h4>The inflammation and cellular dysfunction in periodontitis largely depend on N6-methyladenosine (m⁶A) modification-related epigenetics. However, the role of methyltransferase-like 3 (METTL3)-differentiated m⁶A modification of desmocollin 1 (DSC1) in periodontitis remains to be investigated.<h4>Methods</h4>Periodontal ligament (PDL) tissues were collected from patients with periodontitis and healthy volunteers, and a periodontitis model was constructed using lipopolysaccharide (LPS) and human periodontal ligament fibroblasts (HPLF). METTL3 and DSC1 levels in the specimens and cells were measured using quantitative reverse-transcription polymerase chain reaction. Proliferation, apoptosis, and inflammatory changes were analyzed using CCK-8, EdU, flow cInternational dental journal109347https://www.ebi.ac.uk/biostudies/studies/S-EPMC12800397biostudies-literatureMETTL3-Mediated m6A Modification of DSC1 Alleviates Inflammation and Cellular Dysfunction in Periodontitis.PMC12800397Su QChen JfalseMETTL3-Mediated m6A Modification of DSC1 Alleviates Inflammation and Cellular Dysfunction in Periodontitis.<h4>Background</h4>The inflammation and cellular dysfunction in periodontitis largely depend on N6-methyladenosine (m⁶A) modification-related epigenetics. However, the role of methyltransferase-like 3 (METTL3)-differentiated m⁶A modification of desmocollin 1 (DSC1) in periodontitis remains to be investigated.<h4>Methods</h4>Periodontal ligament (PDL) tissues were collected from patients with periodontitis and healthy volunteers, and a periodontitis model was constructed using lipopolysaccharide (LPS) and human periodontal ligament fibroblasts (HPLF). METTL3 and DSC1 levels in the specimens and cells were measured using quantitative reverse-transcription polymerase chain reaction. Proliferation, apoptosis, and inflammatory changes were analyzed using CCK-8, EdU, flow c2026-01-01T00:00:00Z2026 Feb2026-06-06T14:56:16.007Z2026-06-01T03:08:10.052ZS-EPMC128003974145328610.1016/j.identj.2025.109347