biostudies-literatureKober MGeneralitat de CatalunyaH2020 Marie Sklodowska-Curie ActionsInstituto de Salud Carlos IIIHorizon 2020 Framework ProgrammeAgencia Estatal de InvestigaciónMinisterio de Educación, Cultura y DeporteEuropean Regional Development Fund354-364https://www.ebi.ac.uk/biostudies/studies/S-EPMC12801183biostudies-literatureUnknown12(1)Delivering plasmid DNA (pDNA) into cells is essential for numerous biotechnological and biomedical applications. Among available nanocarriers, nonviral lipid-based vesicles are particularly promising for transfecting mammalian cells. Nevertheless, further development is required to create delivery systems that are both broadly effective across cell types and scalable for clinical use. Here, we explore stable nanovesicles composed of the sterol derivative cholesteryl <i>N</i>-(2-dimethylaminoethyl)carbamate (DC-CHOL) and myristalkonium chloride (MKC) as a platform for pDNA delivery. These nanovesicles, previously shown to efficiently deliver small RNAs to neuroblastoma cells, exhibit favorable physicochemical properties, such as high morphological uniformity and long-term colloidal stabilitACS biomaterials science & engineeringPlasmid DNA Delivery Using a Stable Nanovesicle Platform: A Design-of-Experiments-Guided Investigation.PMC12801183712949CEX2019-000917-SPI23/01144FORT23/00034PID2022-137332OB-I00ICI21000/76CB06/01/00332021-SGR-004382021-SGR-00638FPU16/02555FPU15/03577953110CEX2023-001263-SVentosa NGonzalez-Dominguez IPuente-Massaguer EAviles-Dominguez LSegura MFKober MBoloix AGodia FValdospinos DPique-Ponti JPina DfalsePlasmid DNA Delivery Using a Stable Nanovesicle Platform: A Design-of-Experiments-Guided Investigation.Delivering plasmid DNA (pDNA) into cells is essential for numerous biotechnological and biomedical applications. Among available nanocarriers, nonviral lipid-based vesicles are particularly promising for transfecting mammalian cells. Nevertheless, further development is required to create delivery systems that are both broadly effective across cell types and scalable for clinical use. Here, we explore stable nanovesicles composed of the sterol derivative cholesteryl <i>N</i>-(2-dimethylaminoethyl)carbamate (DC-CHOL) and myristalkonium chloride (MKC) as a platform for pDNA delivery. These nanovesicles, previously shown to efficiently deliver small RNAs to neuroblastoma cells, exhibit favorable physicochemical properties, such as high morphological uniformity and long-term colloidal stabilit2026-01-01T00:00:00Z2026 Jan2026-06-06T15:17:42.669Z2026-06-01T03:11:03.465ZS-EPMC128011834134786610.1021/acsbiomaterials.5c01328