{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lissner R"],"funding":["European Research Council","Dutch Research Council (NWO)","ZonMw"],"pagination":["e0211925"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12802301"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(1)"],"pubmed_abstract":["Accounting for more deaths than any other bacterial species, <i>Mycobacterium tuberculosis</i> (Mtb) represents a critical threat to public health worldwide. A key factor contributing to Mtb's virulence is its unique cell envelope, which acts as a protective barrier. Among the components of this envelope, lipoproteins represent a critical but understudied group of proteins. In this study, we focused on 79 conserved putative lipoproteins, shared between Mtb and the closely related <i>M. marinum</i>. Leveraging the CRISPR/Cas9 gene editing system for Mycobacteria, we generated frameshift mutations, targeting one conserved lipoprotein-coding gene at a time. We identified two mutants, <i>lpqZ</i> and <i>fecB</i>, that exhibited increased susceptibility to all tested antibiotics, suggesting cri"],"journal":["mBio"],"pubmed_title":["A new role for lipoproteins LpqZ and FecB in orchestrating mycobacterial cell envelope biogenesis."],"pmcid":["PMC12802301"],"funding_grant_id":["XL21.006","09120012010040","OCENW.KLEIN.439","832721"],"pubmed_authors":["Lissner R","Benedict ST","Speer A","Franklin A","Knol J","Nejentsev S","Kuijl C","Bitter W","Charitou V","Moynihan PJ","Jimenez C"],"additional_accession":[]},"is_claimable":false,"name":"A new role for lipoproteins LpqZ and FecB in orchestrating mycobacterial cell envelope biogenesis.","description":"Accounting for more deaths than any other bacterial species, <i>Mycobacterium tuberculosis</i> (Mtb) represents a critical threat to public health worldwide. A key factor contributing to Mtb's virulence is its unique cell envelope, which acts as a protective barrier. Among the components of this envelope, lipoproteins represent a critical but understudied group of proteins. In this study, we focused on 79 conserved putative lipoproteins, shared between Mtb and the closely related <i>M. marinum</i>. Leveraging the CRISPR/Cas9 gene editing system for Mycobacteria, we generated frameshift mutations, targeting one conserved lipoprotein-coding gene at a time. We identified two mutants, <i>lpqZ</i> and <i>fecB</i>, that exhibited increased susceptibility to all tested antibiotics, suggesting cri","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-06-06T13:17:34.374Z","creation":"2026-05-31T03:07:14.625Z"},"accession":"S-EPMC12802301","cross_references":{"pubmed":["41384969"],"doi":["10.1128/mbio.02119-25"]}}