{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chacko AN"],"funding":["Institute for Collaborative Biotechnologies","NINDS NIH HHS","National Institutes of Health","Chan Zuckerberg Initiative","NIGMS NIH HHS","National Science Foundation"],"pagination":["eaec1211"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12802846"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(3)"],"pubmed_abstract":["Creating genetic sensors for noninvasive visualization of biological activities in optically opaque tissues holds immense potential for basic research and the development of genetic and cell-based therapies. Magnetic resonance imaging (MRI) stands out among deep tissue imaging methods for its ability to generate high-resolution images without ionizing radiation. However, the adoption of MRI as a mainstream biomolecular technology has been hindered by the lack of adaptable methods to link molecular events with genetically encodable contrast. Here, we introduce modular aquaporin-based protease-activatable probes for enhanced reporting (MAPPER), a platform for the systematic creation of genetic sensors for MRI. To develop MAPPER, we engineered protease-activatable MRI reporters using two appr"],"journal":["Science advances"],"pubmed_title":["A programmable genetic platform for engineering noninvasive biosensors."],"pmcid":["PMC12802846"],"funding_grant_id":["2024-338493","R01 NS128278","R35 GM133530","W911NF-19-D-0001-0009","1625770","R35-GM133530","2308708","R01-NS128278"],"pubmed_authors":["Chacko AN","Pham K","Xu B","Tiwari R","Dhanabalan KM","Wan J","Mukherjee A","Anderson NT","Chien R"],"additional_accession":[]},"is_claimable":false,"name":"A programmable genetic platform for engineering noninvasive biosensors.","description":"Creating genetic sensors for noninvasive visualization of biological activities in optically opaque tissues holds immense potential for basic research and the development of genetic and cell-based therapies. Magnetic resonance imaging (MRI) stands out among deep tissue imaging methods for its ability to generate high-resolution images without ionizing radiation. However, the adoption of MRI as a mainstream biomolecular technology has been hindered by the lack of adaptable methods to link molecular events with genetically encodable contrast. Here, we introduce modular aquaporin-based protease-activatable probes for enhanced reporting (MAPPER), a platform for the systematic creation of genetic sensors for MRI. To develop MAPPER, we engineered protease-activatable MRI reporters using two appr","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-06-11T05:22:42.624Z","creation":"2026-06-11T03:08:24.827Z"},"accession":"S-EPMC12802846","cross_references":{"pubmed":["41533785"],"doi":["10.1126/sciadv.aec1211"]}}