biostudies-literatureNguyen MHCystic Fibrosis FoundationBundesamt für Gesundheit106103https://www.ebi.ac.uk/biostudies/studies/S-EPMC12804157biostudies-literatureUnknown123<h4>Background</h4>Current treatment regimens for nontuberculous mycobacteria (NTM) infections, especially Mycobacterium abscessus, have suboptimal clinical outcomes, demanding novel therapeutic options. The aminoglycoside apramycin (APR) has been suggested as a therapeutic alternative to amikacin (AMK). Here, we report experimental data as part of an ongoing preclinical assessment of APR.<h4>Methods</h4>Antimicrobial activity against 828 isolates comprising the clinically most relevant NTM species were determined by broth microdilution. Bactericidal activity and killing kinetics of APR were determined across a variety of assay conditions against rough and smooth M. abscessus isolates in vitro and in vivo.<h4>Findings</h4>Both the MIC₉₀ and tentative epidemiological cutoff (TECOEBioMedicineMulticentre preclinical profiling of apramycin for the treatment of nontuberculous mycobacteria.PMC12804157JUVABIS22W0HOBBIE19I0Krishnamurthy RVHaldimann KPlattner MBecker KNarayanan SXie MKaur PBulman ZPHobbie SNShandil RKCalado Nogueira de Moura VVerma DSchulthess BSingh MDaley CLMitarai SLang RNguyen MHDick TfalseMulticentre preclinical profiling of apramycin for the treatment of nontuberculous mycobacteria.<h4>Background</h4>Current treatment regimens for nontuberculous mycobacteria (NTM) infections, especially Mycobacterium abscessus, have suboptimal clinical outcomes, demanding novel therapeutic options. The aminoglycoside apramycin (APR) has been suggested as a therapeutic alternative to amikacin (AMK). Here, we report experimental data as part of an ongoing preclinical assessment of APR.<h4>Methods</h4>Antimicrobial activity against 828 isolates comprising the clinically most relevant NTM species were determined by broth microdilution. Bactericidal activity and killing kinetics of APR were determined across a variety of assay conditions against rough and smooth M. abscessus isolates in vitro and in vivo.<h4>Findings</h4>Both the MIC₉₀ and tentative epidemiological cutoff (TECO2026-01-01T00:00:00Z2026 Jan2026-06-06T16:07:51.392Z2026-06-02T03:11:14.511ZS-EPMC128041574147826010.1016/j.ebiom.2025.106103