{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hartauer M"],"funding":["Defense Threat Reduction Agency","NCRR NIH HHS","NIEHS NIH HHS","NCI NIH HHS","National Institute of General Medical Sciences","NIGMS NIH HHS","NIH HHS"],"pagination":["50"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12804587"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["83(1)"],"pubmed_abstract":["There is growing demand for improved in vitro liver models to better predict in vivo pharmacology, specifically drug disposition mediated by hepatic transporters and assessment of transporter-mediated drug interaction risk. While 2D sandwich-cultured human hepatocytes (SCHH) remain valuable, they are limited to short-term use due to hepatocyte de-differentiation and absence of non-parenchymal cells. Multicellular hepatic spheroids (MHS) offer a promising alternative, but transporter concentrations, functionality, and suitability for hepatobiliary transport studies remain unclear. We evaluated an all-human MHS model, comprised of transporter-certified™ cryopreserved primary human hepatocytes (PHH), Kupffer, stellate, and endothelial cells, for long-term hepatic transporter assessment. Over "],"journal":["Cellular and molecular life sciences : CMLS"],"pubmed_title":["Evaluation of a human 3D multicellular hepatic spheroid model as a platform for studying hepatic transporters."],"pmcid":["PMC12804587"],"funding_grant_id":["R35 GM122576","P30 CA016086","S10 OD032350","P30 ES010126","HDTRA1-19-1-0013","P42 ES031007","S10 RR024595"],"pubmed_authors":["Bishop CE","Fallon JK","Wan M","Murphy WA","Brouwer KLR","Hartauer M","Ho H","Tiley JB"],"additional_accession":[]},"is_claimable":false,"name":"Evaluation of a human 3D multicellular hepatic spheroid model as a platform for studying hepatic transporters.","description":"There is growing demand for improved in vitro liver models to better predict in vivo pharmacology, specifically drug disposition mediated by hepatic transporters and assessment of transporter-mediated drug interaction risk. While 2D sandwich-cultured human hepatocytes (SCHH) remain valuable, they are limited to short-term use due to hepatocyte de-differentiation and absence of non-parenchymal cells. Multicellular hepatic spheroids (MHS) offer a promising alternative, but transporter concentrations, functionality, and suitability for hepatobiliary transport studies remain unclear. We evaluated an all-human MHS model, comprised of transporter-certified™ cryopreserved primary human hepatocytes (PHH), Kupffer, stellate, and endothelial cells, for long-term hepatic transporter assessment. Over ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-13T04:49:12.609Z","creation":"2026-06-13T03:09:10.059Z"},"accession":"S-EPMC12804587","cross_references":{"pubmed":["41467945"],"doi":["10.1007/s00018-025-05996-z"]}}