{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["70(7)"],"submitter":["Dacheux D"],"pubmed_abstract":["Pseudomonas aeruginosa clinical cystic fibrosis isolate CHA was mutagenized with Tn5Tc to identify new genes involved in type III secretion system (TTSS)-dependent cytotoxicity toward human polymorphonuclear neutrophils. Among 25 mutants affected in TTSS function, 14 contained the insertion at different positions in the aceAB operon encoding the PDH-E1 and -E2 subunits of pyruvate dehydrogenase. In PDH mutants, no transcriptional activation of TTSS genes in response to calcium depletion occurred. Expression in trans of ExsA restored TTSS function and cytotoxicity."],"journal":["Infection and immunity"],"pagination":["3973-7"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC128050"],"repository":["biostudies-literature"],"pubmed_title":["Activation of the Pseudomonas aeruginosa type III secretion system requires an intact pyruvate dehydrogenase aceAB operon."],"pmcid":["PMC128050"],"pubmed_authors":["de Groot A","Leberre R","Toussaint B","Guery B","Epaulard O","Dacheux D","Polack B","Attree I"],"additional_accession":[]},"is_claimable":false,"name":"Activation of the Pseudomonas aeruginosa type III secretion system requires an intact pyruvate dehydrogenase aceAB operon.","description":"Pseudomonas aeruginosa clinical cystic fibrosis isolate CHA was mutagenized with Tn5Tc to identify new genes involved in type III secretion system (TTSS)-dependent cytotoxicity toward human polymorphonuclear neutrophils. Among 25 mutants affected in TTSS function, 14 contained the insertion at different positions in the aceAB operon encoding the PDH-E1 and -E2 subunits of pyruvate dehydrogenase. In PDH mutants, no transcriptional activation of TTSS genes in response to calcium depletion occurred. Expression in trans of ExsA restored TTSS function and cytotoxicity.","dates":{"release":"2002-01-01T00:00:00Z","publication":"2002 Jul","modification":"2025-04-05T16:17:01.611Z","creation":"2019-03-27T00:17:18Z"},"accession":"S-EPMC128050","cross_references":{"pubmed":["12065547"],"doi":["10.1128/iai.70.7.3973-3977.2002","10.1128/IAI.70.7.3973-3977.2002"]}}