biostudies-literaturePomar CAMinistry of Health of the Czech RepublicMCIN/AEI and ERDF a way of making Europee71452https://www.ebi.ac.uk/biostudies/studies/S-EPMC12805928biostudies-literatureUnknown40(2)Perinatal nutrition influences offspring metabolic phenotype and obesity risk. This study examines how a maternal obesogenic diet and offspring's leptin supplementation during suckling affect the metabolome in offspring adulthood, offering insights into long-term metabolic programming. Offspring of dams fed a standard diet (SD), a Western-style diet (WD) before and during gestation and lactation (WD-dams), or a WD switched to SD during lactation were supplemented throughout suckling with leptin or vehicle and then maintained on an SD post-weaning. Metabolomic analysis of plasma from offspring adult animals revealed sex-specific alterations in the relative abundance of circulating metabolites due to maternal WD. Among these, lower abundance of orotic acid in males and arginine in females emFASEB journal : official publication of the Federation of American Societies for Experimental BiologyMetabolomic Insights Into the Impact of a Maternal Western-Style Diet and Leptin Supplementation During Suckling on Adult Rat Offspring.PMC12805928PID2022-138140NB-I00NU20-07-00026PGC2018-097436-B-I00Palou MPico CPomar CAZouhar PKopecky JCastillo PCajka TPalou AMartin-Chamorro RAfalseMetabolomic Insights Into the Impact of a Maternal Western-Style Diet and Leptin Supplementation During Suckling on Adult Rat Offspring.Perinatal nutrition influences offspring metabolic phenotype and obesity risk. This study examines how a maternal obesogenic diet and offspring's leptin supplementation during suckling affect the metabolome in offspring adulthood, offering insights into long-term metabolic programming. Offspring of dams fed a standard diet (SD), a Western-style diet (WD) before and during gestation and lactation (WD-dams), or a WD switched to SD during lactation were supplemented throughout suckling with leptin or vehicle and then maintained on an SD post-weaning. Metabolomic analysis of plasma from offspring adult animals revealed sex-specific alterations in the relative abundance of circulating metabolites due to maternal WD. Among these, lower abundance of orotic acid in males and arginine in females em2026-01-01T00:00:00Z2026 Jan2026-06-10T05:28:09.038Z2026-06-10T03:07:42.939ZS-EPMC128059284153776010.1096/fj.202501729RRR