biostudies-literatureUnknown19(1)Vazquez de Vasquez MGIR Medtek LLCA fast mid-infrared spectral probe was used to record infrared (IR) spectra of fresh skin tissue removed from patients with biopsy-proven keratinocytic cancer (KC) during Mohs surgery. A 150-patient library of IR spectral data was obtained to see if KC on the epidermal side (outside) of tissue samples can be differentiated from adjacent nonmalignant tissue and normal skin. A linear support vector machine model minimized overtraining predicting the contributions to decisions at each step in the spectrum. Training with an equal cost for KC and nontumor cases samples demonstrated a KC sensitivity of 93% ± 5% and specificity 82% ± 6% (threefold testing esd) on the full library. Success in this test problem warrants future studies to see if differentiation of melanoma from benign melanocytic asJournal of biophotonicse70226https://www.ebi.ac.uk/biostudies/studies/S-EPMC12807626biostudies-literatureEx Vivo Assessment of Keratinocytic Carcinomas Using a Fast Mid-Infrared Probe.PMC12807626Siegle RCasey ASCusick ACoe JVHitchcock CLVazquez de Vasquez MGBradley RCfalseEx Vivo Assessment of Keratinocytic Carcinomas Using a Fast Mid-Infrared Probe.A fast mid-infrared spectral probe was used to record infrared (IR) spectra of fresh skin tissue removed from patients with biopsy-proven keratinocytic cancer (KC) during Mohs surgery. A 150-patient library of IR spectral data was obtained to see if KC on the epidermal side (outside) of tissue samples can be differentiated from adjacent nonmalignant tissue and normal skin. A linear support vector machine model minimized overtraining predicting the contributions to decisions at each step in the spectrum. Training with an equal cost for KC and nontumor cases samples demonstrated a KC sensitivity of 93% ± 5% and specificity 82% ± 6% (threefold testing esd) on the full library. Success in this test problem warrants future studies to see if differentiation of melanoma from benign melanocytic as2026-01-01T00:00:00Z2026 Jan2026-06-10T05:15:10.854Z2026-06-10T03:07:29.13ZS-EPMC128076264153969510.1002/jbio.70226