biostudies-literatureZhou HKey Technologies Research and Development ProgramXihua University103428https://www.ebi.ac.uk/biostudies/studies/S-EPMC12807793biostudies-literatureUnknown33Organophosphate esters (OPEs), as commonly used flame retardant and plasticizer, emerging organic pollutants, exhibit concerning contamination levels, particularly in widely consumed rice-based foods. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of 5 OPEs in rice-based products. According to recoveries and matrix effects as indicators, quality and proportion of different purification fillers were mainly optimized to fabricate a purification cartridge, simplifying and reducing the pre-treatment process and cost. The proposed method showed excellent linearity (0.08-500 μg kg^-1, with all correlation coefficients exceeding 0.99), with improved method detection and quantification limit (MDL and MQL): 0.0047-0.1309 andFood chemistry: XDevelopment and validation of the LC-MS/MS method based on self-made purification column for simultaneous determination of novel organophosphate esters in rice-based products.PMC128077932023YFF1104800Yang XLi ZHuang YCao JQiao DGu MXiao FChen XShao BZhou HfalseDevelopment and validation of the LC-MS/MS method based on self-made purification column for simultaneous determination of novel organophosphate esters in rice-based products.Organophosphate esters (OPEs), as commonly used flame retardant and plasticizer, emerging organic pollutants, exhibit concerning contamination levels, particularly in widely consumed rice-based foods. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of 5 OPEs in rice-based products. According to recoveries and matrix effects as indicators, quality and proportion of different purification fillers were mainly optimized to fabricate a purification cartridge, simplifying and reducing the pre-treatment process and cost. The proposed method showed excellent linearity (0.08-500 μg kg^-1, with all correlation coefficients exceeding 0.99), with improved method detection and quantification limit (MDL and MQL): 0.0047-0.1309 and2026-01-01T00:00:00Z2026 Jan2026-06-10T05:26:00.002Z2026-06-10T03:07:15.459ZS-EPMC128077934155178410.1016/j.fochx.2025.103428