biostudies-literatureShen MEffects of Shenmai Injection on Colorectal Immune Microenvironment and Subsequent Therapeutic Effects30https://www.ebi.ac.uk/biostudies/studies/S-EPMC12808282biostudies-literatureUnknown41(1)<h4>Objectives</h4>This study aims to compare and analyse the immune landscape at the tumour invasion front in patients with colorectal cancer (CRC) with proficient mismatch repair (pMMR) and deficient mismatch repair (dMMR).<h4>Methods</h4>A total of 51 patients with CRC were included, comprising 32 patients with pMMR and 19 patients with dMMR. Immunohistochemistry, fluorescence PCR and capillary electrophoresis were used to detect the expression status of MLH1, PMS2, MSH2 and MSH6 proteins to identify patients with pMMR/MSI-H and pMMR/MSS. Multiplex immunofluorescence technology was employed to stain and analyse immune cells at the tumour invasion front.<h4>Results</h4>In patients with dMMR CRC, the proportion of CD8⁺ T cells at the tumour invasion front was significantly higher than thaInternational journal of colorectal diseaseComparison and analysis of the immune landscape at the tumour invasion front in patients with pMMR/MSI-H and pMMR/MSS colorectal cancer.PMC12808282No.2024ZYC-B82Shen MZhang YRen YChen GCai FShi JfalseComparison and analysis of the immune landscape at the tumour invasion front in patients with pMMR/MSI-H and pMMR/MSS colorectal cancer.<h4>Objectives</h4>This study aims to compare and analyse the immune landscape at the tumour invasion front in patients with colorectal cancer (CRC) with proficient mismatch repair (pMMR) and deficient mismatch repair (dMMR).<h4>Methods</h4>A total of 51 patients with CRC were included, comprising 32 patients with pMMR and 19 patients with dMMR. Immunohistochemistry, fluorescence PCR and capillary electrophoresis were used to detect the expression status of MLH1, PMS2, MSH2 and MSH6 proteins to identify patients with pMMR/MSI-H and pMMR/MSS. Multiplex immunofluorescence technology was employed to stain and analyse immune cells at the tumour invasion front.<h4>Results</h4>In patients with dMMR CRC, the proportion of CD8⁺ T cells at the tumour invasion front was significantly higher than tha2026-01-01T00:00:00Z2026 Jan2026-06-06T15:36:45.227Z2026-06-02T03:08:39.295ZS-EPMC128082824153557710.1007/s00384-025-05033-2