biostudies-literatureUnknown13Salzmann RJSHuman-derived biomaterials offer several advantages over animal-derived or synthetic alternatives, including improved biocompatibility, ethical acceptability, sustainability, and clinical translatability. Here we present new applications of human placenta-derived materials - specifically HUMAN PLACENTA substrate, collagen type-I, and Laminin-111 - as 2D coating materials and 3D matrices for the cultivation of spheroids and adherent cells. Collagen type-I coatings supported colorectal cancer spheroid formation without the need for growth-factor supplementation. Lm-111 significantly enhanced NIH3T3 fibroblast adhesion compared with poly-L-lysine and rat-tail collagen type-I, performing comparably to bovine fibronectin. In a transwell blood-brain barrier model, HUMAN PLACENTA substrate coatinFrontiers in bioengineering and biotechnology1676369https://www.ebi.ac.uk/biostudies/studies/S-EPMC12808481biostudies-literatureNew applications of sustainable, scalable, standardized, and cost-effective human biomaterials for cell-based assays, tissue engineering, and regenerative medicine.PMC12808481Moroni LSalzmann RJSKigili SNeuhaus WHackethal JEgger GPrado-Lopez SDomazet SBrachner AfalseNew applications of sustainable, scalable, standardized, and cost-effective human biomaterials for cell-based assays, tissue engineering, and regenerative medicine.Human-derived biomaterials offer several advantages over animal-derived or synthetic alternatives, including improved biocompatibility, ethical acceptability, sustainability, and clinical translatability. Here we present new applications of human placenta-derived materials - specifically HUMAN PLACENTA substrate, collagen type-I, and Laminin-111 - as 2D coating materials and 3D matrices for the cultivation of spheroids and adherent cells. Collagen type-I coatings supported colorectal cancer spheroid formation without the need for growth-factor supplementation. Lm-111 significantly enhanced NIH3T3 fibroblast adhesion compared with poly-L-lysine and rat-tail collagen type-I, performing comparably to bovine fibronectin. In a transwell blood-brain barrier model, HUMAN PLACENTA substrate coatin2025-01-01T00:00:00Z20252026-06-12T04:54:09.722Z2026-06-12T03:08:02.546ZS-EPMC128084814155037110.3389/fbioe.2025.1676369