{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Griffiths R"],"funding":["Biotechnology and Biological Sciences Research Council"],"pagination":["708-717"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12809243"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["81(Pt 12)"],"pubmed_abstract":["The accessory secretion (aSec) system is a protein export pathway that is uniquely present in Gram-positive bacteria and is dedicated to the secretion of large, glycosylated cell wall-anchored adhesins called serine-rich repeat proteins (SRRPs). Strain-specific glycosylation of SRRPs has previously been reported in Limosilactobacillus reuteri and attributed to GtfC, a glycosyltransferase belonging to family 113, with LrGtfC<sub>100-23</sub> from L. reuteri rat strain 100-23C showing specificity for UDP-Glc, while LrGtfC<sub>53608</sub> from L. reuteri pig strain ATCC 53608, which differs at only ten amino-acid positions, shows a preference for UDP-GlcNAc. However, the structural basis underpinning GtfC sugar-donor specificity remains unclear. Here, we report X-ray crystal structures of the"],"journal":["Acta crystallographica. Section D, Structural biology"],"pubmed_title":["A structural basis for the strain-dependent UDP-sugar specificity of glycosyltransferase C from the Limosilactobacillus reuteri accessory secretion system."],"pmcid":["PMC12809243"],"funding_grant_id":["BB/R012490/1","BB/X011054/1","BB/M011216/1"],"pubmed_authors":["Juge N","Griffiths R","Ashworth G","Hemmings A","Pfalzgraf H","Dong C","Latousakis D"],"additional_accession":[]},"is_claimable":false,"name":"A structural basis for the strain-dependent UDP-sugar specificity of glycosyltransferase C from the Limosilactobacillus reuteri accessory secretion system.","description":"The accessory secretion (aSec) system is a protein export pathway that is uniquely present in Gram-positive bacteria and is dedicated to the secretion of large, glycosylated cell wall-anchored adhesins called serine-rich repeat proteins (SRRPs). Strain-specific glycosylation of SRRPs has previously been reported in Limosilactobacillus reuteri and attributed to GtfC, a glycosyltransferase belonging to family 113, with LrGtfC<sub>100-23</sub> from L. reuteri rat strain 100-23C showing specificity for UDP-Glc, while LrGtfC<sub>53608</sub> from L. reuteri pig strain ATCC 53608, which differs at only ten amino-acid positions, shows a preference for UDP-GlcNAc. However, the structural basis underpinning GtfC sugar-donor specificity remains unclear. Here, we report X-ray crystal structures of the","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-06T16:02:40.725Z","creation":"2026-06-02T03:09:49.864Z"},"accession":"S-EPMC12809243","cross_references":{"pubmed":["41190658"],"doi":["10.1107/S2059798325008782"]}}