biostudies-literatureIftehimul MNINDS NIH HHSNational Institutes of HealthNorth Carolina A&amp;amp;T State UniversityNIGMS NIH HHS169-178https://www.ebi.ac.uk/biostudies/studies/S-EPMC12809272biostudies-literatureUnknown31<h4>Background</h4>The triple-negative breast cancer (TNBC) microenvironment undergoes progressive reprogramming, transitioning from an early immune-active state to a late immune-suppressed state. While tumor cell plasticity has been extensively studied, the temporal molecular remodeling of T cells <i>in vivo</i> remains poorly defined.<h4>Results</h4>Transcriptional analysis of T cells within 4T1 TNBC tumors, harvested at one-, three-, and six-weeks post-tumor implantation in the mammary fat pads of BALB/c mice, revealed a decline in transcriptomic signatures associated with T cells from 194 at one week to 156 at six weeks, with a significant late-stage loss or reduction of transcripts related to T cell receptors (TCR), natural killer T, and gamma delta T cells. Furthermore, changes in vaComputational and structural biotechnology journalTemporal molecular remodeling of T cells informs their possible adaptation in 4T1 tumors.PMC12809272R35 GM153737R16 NS147983Kaufman HLMuganda PMSaha DNewman RHRorie CJGraves JLJones RBIftehimul MHarrison SHHolloman BLThomas MDHossain MTfalseTemporal molecular remodeling of T cells informs their possible adaptation in 4T1 tumors.<h4>Background</h4>The triple-negative breast cancer (TNBC) microenvironment undergoes progressive reprogramming, transitioning from an early immune-active state to a late immune-suppressed state. While tumor cell plasticity has been extensively studied, the temporal molecular remodeling of T cells <i>in vivo</i> remains poorly defined.<h4>Results</h4>Transcriptional analysis of T cells within 4T1 TNBC tumors, harvested at one-, three-, and six-weeks post-tumor implantation in the mammary fat pads of BALB/c mice, revealed a decline in transcriptomic signatures associated with T cells from 194 at one week to 156 at six weeks, with a significant late-stage loss or reduction of transcripts related to T cell receptors (TCR), natural killer T, and gamma delta T cells. Furthermore, changes in va2026-01-01T00:00:00Z20262026-07-15T06:35:05.077Z2026-06-30T03:21:44.384ZS-EPMC128092724155013710.1016/j.csbj.2025.12.022