{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["6(4)"],"submitter":["Verbunt J"],"pubmed_abstract":["<b>Aim:</b> This study aimed to evaluate the reproducibility of the isolation and characterization of feces-derived bacterial membrane vesicles. <b>Methods:</b> Human fecal samples (<i>n</i> = 12) stored at -80 °C were thawed, sampled, and then refrozen. From these samples, bacterial membrane vesicles were isolated through ultrafiltration, ultracentrifugation and size exclusion chromatography. Vesicle-associated DNA was characterized by marker [16 ribosomal DNA (rDNA)] sequencing to determine composition. The same fecal samples were thawed again after > 6 months of storage at -80 °C to repeat this procedure. Compositions and other vesicle characteristics were compared to investigate effects of storage and freeze/thawing on sample stability. In addition, for four of the fecal aliquots, the "],"journal":["Extracellular vesicles and circulating nucleic acids"],"pagination":["728-741"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12809393"],"repository":["biostudies-literature"],"pubmed_title":["Assessment of the reproducibility of bacterial membrane vesicle isolation and characterization."],"pmcid":["PMC12809393"],"pubmed_authors":["Blaak EE","Canfora E","Barnett D","Stassen F","Savelkoul P","Jocken J","Verbunt J"],"additional_accession":[]},"is_claimable":false,"name":"Assessment of the reproducibility of bacterial membrane vesicle isolation and characterization.","description":"<b>Aim:</b> This study aimed to evaluate the reproducibility of the isolation and characterization of feces-derived bacterial membrane vesicles. <b>Methods:</b> Human fecal samples (<i>n</i> = 12) stored at -80 °C were thawed, sampled, and then refrozen. From these samples, bacterial membrane vesicles were isolated through ultrafiltration, ultracentrifugation and size exclusion chromatography. Vesicle-associated DNA was characterized by marker [16 ribosomal DNA (rDNA)] sequencing to determine composition. The same fecal samples were thawed again after > 6 months of storage at -80 °C to repeat this procedure. Compositions and other vesicle characteristics were compared to investigate effects of storage and freeze/thawing on sample stability. In addition, for four of the fecal aliquots, the ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-06-06T14:40:11.878Z","creation":"2026-05-31T03:10:15.639Z"},"accession":"S-EPMC12809393","cross_references":{"pubmed":["41551598"],"doi":["10.20517/evcna.2025.71"]}}