biostudies-literatureUnknown9(1)Jin WInner Mongolia Science & Technology Plan<h4>Background</h4>Chemotherapy resistance in colorectal cancer (CRC) is often mediated by enhanced DNA damage repair (DDR). Transmembrane protein TMEM59L is implicated in cancer progression, but its role in CRC chemoresistance is unclear. We investigated whether TMEM59L regulates 5-fluorouracil (5-FU) sensitivity through PTPRN-mediated DDR.<h4>Aims</h4>This study aimed to investigate the role of TMEM59L in regulating PTPRN-mediated DNA damage repair and its impact on 5-FU sensitivity in colorectal cancer, with the goal of identifying potential therapeutic targets to overcome chemoresistance.<h4>Methods and results</h4>Bioinformatics analyses assessed TMEM59L/PTPRN expression and prognosis in CRC cohorts. Gain- and loss-of-function experiments were conducted in CRC cell lines (HCT116, SW48Cancer reports (Hoboken, N.J.)e70448https://www.ebi.ac.uk/biostudies/studies/S-EPMC12809716biostudies-literatureTransmembrane Protein TMEM59L Modulates 5-FU Resistance via PTPRN-Mediated DNA Damage Repair in Colorectal Cancer.PMC12809716Ma QJin WHou JKang HWang CWang FMa ZWang XLiu FfalseTransmembrane Protein TMEM59L Modulates 5-FU Resistance via PTPRN-Mediated DNA Damage Repair in Colorectal Cancer.<h4>Background</h4>Chemotherapy resistance in colorectal cancer (CRC) is often mediated by enhanced DNA damage repair (DDR). Transmembrane protein TMEM59L is implicated in cancer progression, but its role in CRC chemoresistance is unclear. We investigated whether TMEM59L regulates 5-fluorouracil (5-FU) sensitivity through PTPRN-mediated DDR.<h4>Aims</h4>This study aimed to investigate the role of TMEM59L in regulating PTPRN-mediated DNA damage repair and its impact on 5-FU sensitivity in colorectal cancer, with the goal of identifying potential therapeutic targets to overcome chemoresistance.<h4>Methods and results</h4>Bioinformatics analyses assessed TMEM59L/PTPRN expression and prognosis in CRC cohorts. Gain- and loss-of-function experiments were conducted in CRC cell lines (HCT116, SW482026-01-01T00:00:00Z2026 Jan2026-06-10T05:27:19.601Z2026-06-10T03:07:28.391ZS-EPMC128097164154304610.1002/cnr2.70448