biostudies-literatureTakacs JDutch Research Council (NWO)100121https://www.ebi.ac.uk/biostudies/studies/S-EPMC12814073biostudies-literatureUnknown9The house cricket, <i>Acheta domesticus,</i> is commonly reared for food and feed purposes and is often infected with the Acheta domesticus densovirus (AdDV). This single-stranded DNA virus can cause high mortality in crickets resulting in colony collapse. AdDV disease outbreaks in cricket mass-rearing can be prevented by either obtaining virus-free crickets or by reducing virus spread. Therefore, insight into viral levels in cricket developmental stages and into viral transmission routes is needed. Viral levels were monitored using quantitative PCR on samples collected 1) simultaneously from different life stages present in the rearing room and 2) weekly from a single rearing container during the successive developmental stages. To study viral tissue tropism and to infer the route of viruCurrent research in insect scienceTissue tropism and viral levels of Acheta domesticus densovirus throughout the house cricket production.PMC12814073VI.Vidi.192.041Jensen ABTakacs JBryon ARos VIDvan Loon JJAfalseTissue tropism and viral levels of Acheta domesticus densovirus throughout the house cricket production.The house cricket, <i>Acheta domesticus,</i> is commonly reared for food and feed purposes and is often infected with the Acheta domesticus densovirus (AdDV). This single-stranded DNA virus can cause high mortality in crickets resulting in colony collapse. AdDV disease outbreaks in cricket mass-rearing can be prevented by either obtaining virus-free crickets or by reducing virus spread. Therefore, insight into viral levels in cricket developmental stages and into viral transmission routes is needed. Viral levels were monitored using quantitative PCR on samples collected 1) simultaneously from different life stages present in the rearing room and 2) weekly from a single rearing container during the successive developmental stages. To study viral tissue tropism and to infer the route of viru2026-01-01T00:00:00Z20262026-07-15T13:49:25.143Z2026-07-05T03:11:07.551ZS-EPMC128140734156180810.1016/j.cris.2025.100121