{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mereness JA"],"funding":["NEI NIH HHS","University of Rochester","NIEHS NIH HHS","National Institutes of Health","NIAMS NIH HHS"],"pagination":["117771"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12815593"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["205"],"pubmed_abstract":["Bone autograft healing is a highly orchestrated process that integrates immune activation, vascular ingrowth, and osteogenic remodeling. To define the molecular and cellular programs driving early autograft integration, bulk and single-cell RNA sequencing was used to analyze graft-associated tissues over 14 days in a murine periosteal-mediated autograft model. Global transcriptomic analysis revealed rapid and dynamic remodeling, with maximal gene expression changes occurring within the first week. The first 48 h were dominated by pro-inflammatory signaling, including TNF, IL-1, TLR, and MAPK pathways, accompanied by transcriptional signatures of phagocytosis and cellular clearance. These early inflammatory programs gave way to pro-regenerative signals, including activation of HIF-1, PI3K-A"],"journal":["Bone"],"pubmed_title":["Temporal transcriptomic profiling of bone autograft healing reveals dynamic immune, vascular, and osteogenic programs."],"pmcid":["PMC12815593"],"funding_grant_id":["T32 ES007026","R01 EY033192","R01 AR064200","R21 EY030817"],"pubmed_authors":["Benoit DSW","Mereness JA","Basu S","Benoodt L"],"additional_accession":[]},"is_claimable":false,"name":"Temporal transcriptomic profiling of bone autograft healing reveals dynamic immune, vascular, and osteogenic programs.","description":"Bone autograft healing is a highly orchestrated process that integrates immune activation, vascular ingrowth, and osteogenic remodeling. To define the molecular and cellular programs driving early autograft integration, bulk and single-cell RNA sequencing was used to analyze graft-associated tissues over 14 days in a murine periosteal-mediated autograft model. Global transcriptomic analysis revealed rapid and dynamic remodeling, with maximal gene expression changes occurring within the first week. The first 48 h were dominated by pro-inflammatory signaling, including TNF, IL-1, TLR, and MAPK pathways, accompanied by transcriptional signatures of phagocytosis and cellular clearance. These early inflammatory programs gave way to pro-regenerative signals, including activation of HIF-1, PI3K-A","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-12T05:11:17.712Z","creation":"2026-06-12T03:07:45.513Z"},"accession":"S-EPMC12815593","cross_references":{"pubmed":["41478392"],"doi":["10.1016/j.bone.2025.117771"]}}