{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16"],"submitter":["Ramesh AI"],"pubmed_abstract":["<h4>Background</h4><i>Cryptococcus neoformans</i> causes lethal meningoencephalitis and long-term neurological deficits, particularly in immunocompromised hosts of any age group. After penetrating the airway and crossing the blood-tissue barriers, <i>C. neoformans</i> rapidly enters the brain, where it extensively releases the capsular polysaccharide glucuronoxylomannan (GXM), a major virulence factor.<h4>Methods</h4><i>Cryptococcus neoformans (</i>ATCC 32045) was used to isolate and purify GXM. This study utilized human-induced pluripotent stem cell-derived 2D cultured neural stem cells, neurons, and microglia-deficient cerebral organoids to identify GXM-induced pathogenesis. Cryosectioning of frozen tissues, immunostaining, western blot analysis and untargeted lipidomics were employed to"],"journal":["Frontiers in immunology"],"pagination":["1687068"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12815873"],"repository":["biostudies-literature"],"pubmed_title":["Host-membrane lipid composition controls &lt;i&gt;Cryptococcus neoformans&lt;/i&gt; cellular targets."],"pmcid":["PMC12815873"],"pubmed_authors":["Reddy SK","Bhat V","Krishnamurthy S","Mariappan I","Hegde S","Shetty AK","Mallikarjuna PV","Shettigar KS","Chandran D","Ramesh AI","Bhat P","Yegneswaran PP","Saravu K","Dabhi AM","Sabitha KR","Aimanianda V","Upadhya D"],"additional_accession":[]},"is_claimable":false,"name":"Host-membrane lipid composition controls &lt;i&gt;Cryptococcus neoformans&lt;/i&gt; cellular targets.","description":"<h4>Background</h4><i>Cryptococcus neoformans</i> causes lethal meningoencephalitis and long-term neurological deficits, particularly in immunocompromised hosts of any age group. After penetrating the airway and crossing the blood-tissue barriers, <i>C. neoformans</i> rapidly enters the brain, where it extensively releases the capsular polysaccharide glucuronoxylomannan (GXM), a major virulence factor.<h4>Methods</h4><i>Cryptococcus neoformans (</i>ATCC 32045) was used to isolate and purify GXM. This study utilized human-induced pluripotent stem cell-derived 2D cultured neural stem cells, neurons, and microglia-deficient cerebral organoids to identify GXM-induced pathogenesis. Cryosectioning of frozen tissues, immunostaining, western blot analysis and untargeted lipidomics were employed to","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-06-12T05:18:47.174Z","creation":"2026-06-12T03:07:49.934Z"},"accession":"S-EPMC12815873","cross_references":{"pubmed":["41567201"],"doi":["10.3389/fimmu.2025.1687068"]}}