biostudies-literatureUnknown16(1)Joshi MKumaun University, NainitalPlant-based products have emerged as a high-rise need. Thymus linearis, a hilly herb, represents a promising resource of natural bioactive compounds. This work targets multiple impacts of essential oil of T. linearis, examining its chemical variability across different geographical regions, giving a GC-MS-based metabolite profile of T. linearis essential oil, along with tests done in vitro and in silico to see how well it works as an antioxidant (DPPH, ABTS, and metal chelating activity), antidiabetic (α-amylase assay), and antibacterial (disc-diffusion against E. coli and B. subtilis) agent. The essential oil was extracted via hydrodistillation from three locations: Ranikhet (RTL), Almora (ATL), and Lohaghat (LTL). Thymol (61.95% to 73.05%) was dominant in all three locations, with RTL beScientific reports2494https://www.ebi.ac.uk/biostudies/studies/S-EPMC12820053biostudies-literatureMetabolite profiling and evaluation of antioxidant, antidiabetic, and antibacterial potential of Thymus linearis Benth. supported by molecular docking and PASS prediction.PMC12820053Joshi MDuseja MTewari LMPande CBachhar VKharkwal GCJoshi VTewari GGangwar AfalseMetabolite profiling and evaluation of antioxidant, antidiabetic, and antibacterial potential of Thymus linearis Benth. supported by molecular docking and PASS prediction.Plant-based products have emerged as a high-rise need. Thymus linearis, a hilly herb, represents a promising resource of natural bioactive compounds. This work targets multiple impacts of essential oil of T. linearis, examining its chemical variability across different geographical regions, giving a GC-MS-based metabolite profile of T. linearis essential oil, along with tests done in vitro and in silico to see how well it works as an antioxidant (DPPH, ABTS, and metal chelating activity), antidiabetic (α-amylase assay), and antibacterial (disc-diffusion against E. coli and B. subtilis) agent. The essential oil was extracted via hydrodistillation from three locations: Ranikhet (RTL), Almora (ATL), and Lohaghat (LTL). Thymol (61.95% to 73.05%) was dominant in all three locations, with RTL be2025-01-01T00:00:00Z2025 Dec2026-06-06T19:40:16.782Z2026-06-04T03:11:35.618ZS-EPMC128200534139218610.1038/s41598-025-32317-y