{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Leng F"],"funding":["Howard Hughes Medical Institute","NIAID NIH HHS","NIH"],"pagination":["116633"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12820563"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["44(12)"],"pubmed_abstract":["FoxP3, the master regulator of Tregs, employs two DNA-binding modes to recognize diverse DNA sequences. It multimerizes on long TnG repeats (n = 2-5) to bridge DNA segments and stabilize chromatin loops, and it forms head-to-head (H-H) dimers on inverted repeat forkhead motifs (IR-FKHM) without bridging DNA. Although genomic data confirm its multimeric role, in vivo evidence for H-H dimerization has been elusive. Here, unbiased pull-down sequencing uncovers a range of relaxed motifs that drive H-H dimerization, enabling systematic genome-wide analysis. We demonstrate that FoxP3 binds genomic DNA as both H-H dimers and multimers in Tregs, with H-H binding often seeding and stabilizing multimerization on adjacent TnG repeats-especially on shorter, suboptimal repeats. While multimerization is"],"journal":["Cell reports"],"pubmed_title":["FoxP3 forms a head-to-head dimer in vivo and stabilizes its multimerization on adjacent microsatellites."],"pmcid":["PMC12820563"],"funding_grant_id":["R01 AI180137","R01AI180137"],"pubmed_authors":["Zhang W","Viennet T","Hur S","Wang C","Clark R","Leng F","Wang X","Arthanari H"],"additional_accession":[]},"is_claimable":false,"name":"FoxP3 forms a head-to-head dimer in vivo and stabilizes its multimerization on adjacent microsatellites.","description":"FoxP3, the master regulator of Tregs, employs two DNA-binding modes to recognize diverse DNA sequences. It multimerizes on long TnG repeats (n = 2-5) to bridge DNA segments and stabilize chromatin loops, and it forms head-to-head (H-H) dimers on inverted repeat forkhead motifs (IR-FKHM) without bridging DNA. Although genomic data confirm its multimeric role, in vivo evidence for H-H dimerization has been elusive. Here, unbiased pull-down sequencing uncovers a range of relaxed motifs that drive H-H dimerization, enabling systematic genome-wide analysis. We demonstrate that FoxP3 binds genomic DNA as both H-H dimers and multimers in Tregs, with H-H binding often seeding and stabilizing multimerization on adjacent TnG repeats-especially on shorter, suboptimal repeats. While multimerization is","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-06T19:23:35.002Z","creation":"2026-06-04T03:12:58.931Z"},"accession":"S-EPMC12820563","cross_references":{"pubmed":["41313681"],"doi":["10.1016/j.celrep.2025.116633"]}}