biostudies-literatureYu HPostgraduates &apos; innovative scientific research project of colleges and universities in Hainan ProvincePostgraduates ' innovative scientific research project of colleges and universities in Hainan Province96https://www.ebi.ac.uk/biostudies/studies/S-EPMC12821990biostudies-literatureUnknown26(1)<h4>Background</h4>Cytokeratin 19 (CK19), Ki67 antigen (Ki67), and β-catenin are abnormally overexpressed in hepatocellular carcinoma (HCC), but their diagnostic and prognostic value remains unclear. This study aims to investigate the predictive role of these three markers in post-operative survival of HCC patients.<h4>Methods</h4>The expression levels of CK19, Ki67, and β-catenin in HCC tumor tissues were determined through public datasets. Kaplan-Meier survival analysis and multivariate Cox regression were performed to evaluate their prognostic value. Immunohistochemistry and Western blotting were used to detect the expression levels of CK19, Ki67, and β-catenin in hepatocellular carcinoma tissues and adjacent non-cancerous tissues. Transcriptome sequencing was performed to analyze the dBMC cancerElevated expression of CK19, Ki67, and β-Catenin as prognostic biomarkers in hepatocellular carcinoma.PMC12821990Qhys2023-498Wu JGao LYu HfalseElevated expression of CK19, Ki67, and β-Catenin as prognostic biomarkers in hepatocellular carcinoma.<h4>Background</h4>Cytokeratin 19 (CK19), Ki67 antigen (Ki67), and β-catenin are abnormally overexpressed in hepatocellular carcinoma (HCC), but their diagnostic and prognostic value remains unclear. This study aims to investigate the predictive role of these three markers in post-operative survival of HCC patients.<h4>Methods</h4>The expression levels of CK19, Ki67, and β-catenin in HCC tumor tissues were determined through public datasets. Kaplan-Meier survival analysis and multivariate Cox regression were performed to evaluate their prognostic value. Immunohistochemistry and Western blotting were used to detect the expression levels of CK19, Ki67, and β-catenin in hepatocellular carcinoma tissues and adjacent non-cancerous tissues. Transcriptome sequencing was performed to analyze the d2025-01-01T00:00:00Z2025 Dec2026-06-06T19:29:45.828Z2026-06-04T03:10:51.362ZS-EPMC128219904138852010.1186/s12885-025-15429-6