biostudies-literatureNiu YFujian Provincial Major Science and Technology ProjectFreely Explore Technological Innovation Projects106https://www.ebi.ac.uk/biostudies/studies/S-EPMC12822208biostudies-literatureUnknown26(1)The production of Polygonatum cyrtonema Hua is currently encountering substantial challenges due to root rot disease, primarily caused by the pathogen Fusarium oxysporum. The WRKY transcription factors play crucial roles in mediating disease resistance across a wide range of plant species; however, it is unclear whether members of this family improve resistance to F. oxysporum infection in P. cyrtonema Hua. To putatively determine the F. oxysporum responsive PcWRKY members, we analyzed full-length transcriptome sequencing data obtained after F. oxysporum infection in this study, 271 WRKY transcription factors were detected and PcWRKY33 was found to be responsive to F. oxysporum infection. Furthermore, overexpression of PcWRKY33 in both P. cyrtonema Hua and Nicotiana benthamiana increased tBMC plant biologyPcWRKY33 enhances Fusarium oxysporum resistance in Polygonatum Cyrtonema Hua via salicylic acid-mediated defense activation.PMC128222082022NZ029017ZYTS202404Zheng HZhu YZheng MTian DChen HLi LSu HXiao RNiu YfalsePcWRKY33 enhances Fusarium oxysporum resistance in Polygonatum Cyrtonema Hua via salicylic acid-mediated defense activation.The production of Polygonatum cyrtonema Hua is currently encountering substantial challenges due to root rot disease, primarily caused by the pathogen Fusarium oxysporum. The WRKY transcription factors play crucial roles in mediating disease resistance across a wide range of plant species; however, it is unclear whether members of this family improve resistance to F. oxysporum infection in P. cyrtonema Hua. To putatively determine the F. oxysporum responsive PcWRKY members, we analyzed full-length transcriptome sequencing data obtained after F. oxysporum infection in this study, 271 WRKY transcription factors were detected and PcWRKY33 was found to be responsive to F. oxysporum infection. Furthermore, overexpression of PcWRKY33 in both P. cyrtonema Hua and Nicotiana benthamiana increased t2025-01-01T00:00:00Z2025 Dec2026-06-04T03:13:10.294Z2026-06-04T03:10:10.719ZS-EPMC128222084140814410.1186/s12870-025-07921-9