{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ghislanzoni S"],"funding":["Italian Ministry of Public Health","European Union's Horizon 2020 research and innovation program"],"pagination":["2678"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12823619"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["Despite advances in cancer therapies, treatment failure from resistance and recurrence remains a major clinical challenge. Therapy-induced senescence (TIS), a state of stable cell cycle arrest with sustained metabolic activity, has emerged as a driver of inflammation, tumor persistence, and relapse. However, the heterogeneity of TIS complicates its detection and targeting. Here, we applied a multi-modal strategy to characterize metabolic alterations in senescent cancer cells induced by doxorubicin or γ-irradiation across three tumor cell lines: MCF7, HeLa, and TPC-1. Mitochondrial dysfunction was assessed using MitoTracker and JC-1 staining, while two-photon excitation fluorescence (TPEF) microscopy enabled label-free visualization of intracellular NAD(P)H and FAD distribution. Lipid remod"],"journal":["Scientific reports"],"pubmed_title":["Metabolic profiling of therapy-induced senescent cancer cells via TPEF, MALDI-MS, and RNA-sequencing."],"pmcid":["PMC12823619"],"funding_grant_id":["101016923","RF 2019-12370456"],"pubmed_authors":["Belfiore A","Niero M","Betti S","Torelli S","Bresci A","Bongarzone I","Bertolotti A","Ghislanzoni S","Padelli F","Agnelli L","Masella A","Polli D"],"additional_accession":[]},"is_claimable":false,"name":"Metabolic profiling of therapy-induced senescent cancer cells via TPEF, MALDI-MS, and RNA-sequencing.","description":"Despite advances in cancer therapies, treatment failure from resistance and recurrence remains a major clinical challenge. Therapy-induced senescence (TIS), a state of stable cell cycle arrest with sustained metabolic activity, has emerged as a driver of inflammation, tumor persistence, and relapse. However, the heterogeneity of TIS complicates its detection and targeting. Here, we applied a multi-modal strategy to characterize metabolic alterations in senescent cancer cells induced by doxorubicin or γ-irradiation across three tumor cell lines: MCF7, HeLa, and TPC-1. Mitochondrial dysfunction was assessed using MitoTracker and JC-1 staining, while two-photon excitation fluorescence (TPEF) microscopy enabled label-free visualization of intracellular NAD(P)H and FAD distribution. Lipid remod","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-13T05:03:00.063Z","creation":"2026-06-13T03:08:40.193Z"},"accession":"S-EPMC12823619","cross_references":{"pubmed":["41408108"],"doi":["10.1038/s41598-025-32573-y"]}}