biostudies-literatureUnknown16Wu M<h4>Background</h4>Colorectal cancer (CRC) remains a leading cause of cancer-related morbidity and mortality worldwide. Cardamonin (CDN), a bioactive flavonoid derived from the seeds of <i>Alpinia katsumadai</i> Hayata, has demonstrated broad-spectrum anticancer potential. However, its specific mechanisms and therapeutic targets in CRC remain poorly elucidated.<h4>Methods</h4>Network pharmacology and molecular docking were employed to identify signaling pathways and targets associated with the anti-CRC activity of CDN. Cell viability, proliferation, migration, and invasion were evaluated using CCK-8, EdU, wound healing, and Transwell assays, respectively. Apoptosis and cell cycle were analyzed by flow cytometry. Proteomic profiling was applied to explore the underlying mechanisms, and the Frontiers in pharmacology1739201https://www.ebi.ac.uk/biostudies/studies/S-EPMC12823903biostudies-literatureCardamonin induces apoptosis of colorectal cancer cells via targeted inhibition of the JAK/STAT3/epithelial-mesenchymal transition (EMT) signaling axis.PMC12823903Wu MLiu ZDu ZChen CRen DLiu WfalseCardamonin induces apoptosis of colorectal cancer cells via targeted inhibition of the JAK/STAT3/epithelial-mesenchymal transition (EMT) signaling axis.<h4>Background</h4>Colorectal cancer (CRC) remains a leading cause of cancer-related morbidity and mortality worldwide. Cardamonin (CDN), a bioactive flavonoid derived from the seeds of <i>Alpinia katsumadai</i> Hayata, has demonstrated broad-spectrum anticancer potential. However, its specific mechanisms and therapeutic targets in CRC remain poorly elucidated.<h4>Methods</h4>Network pharmacology and molecular docking were employed to identify signaling pathways and targets associated with the anti-CRC activity of CDN. Cell viability, proliferation, migration, and invasion were evaluated using CCK-8, EdU, wound healing, and Transwell assays, respectively. Apoptosis and cell cycle were analyzed by flow cytometry. Proteomic profiling was applied to explore the underlying mechanisms, and the 2025-01-01T00:00:00Z20252026-06-21T03:10:24.837Z2026-06-21T03:07:35.581ZS-EPMC128239034158587810.3389/fphar.2025.1739201