{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Berzins U"],"pubmed_abstract":["Younger, replicative cells with longer telomeres can enhance regenerative therapies, however, there is a lack of a standard method to assess telomere length in live cells. The present study investigated whether the relative size of human adipose tissue-derived mesenchymal stromal cells (AD-MSCs) can influence their telomere length. During early culture, a smaller-sized AD-MSC subpopulation was identified based on characteristic colony emergence. Telomere lengths in total and smaller-sized cell populations were measured. Polymerase chain reaction revealed expression of Nanog and OCT3/4 in small-sized AD-MSCs. Their safety was evaluated in immunodeficient BALB/c nude mice. Smaller AD-MSCs revealed distinct growth properties, with the cell monolayer rolling up into a large aggregate. These cells had longer telomeres (18,121.43 base pairs [bp]) than the total population (15,870.44 bp) and formed teratoma-like structures with skin-like morphology (including hair). In conclusion, AD-MSC size reliably isolates cells with longer telomeres and potential."],"journal":["Frontiers in bioengineering and biotechnology"],"pagination":["1687461"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12823908"],"repository":["biostudies-literature"],"pubmed_title":["Small adipose-derived mesenchymal stromal cells exhibit longer telomeres and enhanced regenerative potential."],"pmcid":["PMC12823908"],"pubmed_authors":["Baronins J","Goel S","Rautmane A","Sorokins H","Berzins U","Shishkin A","Zvirgzdina R","Minchev D"],"additional_accession":[]},"is_claimable":false,"name":"Small adipose-derived mesenchymal stromal cells exhibit longer telomeres and enhanced regenerative potential.","description":"Younger, replicative cells with longer telomeres can enhance regenerative therapies, however, there is a lack of a standard method to assess telomere length in live cells. The present study investigated whether the relative size of human adipose tissue-derived mesenchymal stromal cells (AD-MSCs) can influence their telomere length. During early culture, a smaller-sized AD-MSC subpopulation was identified based on characteristic colony emergence. Telomere lengths in total and smaller-sized cell populations were measured. Polymerase chain reaction revealed expression of Nanog and OCT3/4 in small-sized AD-MSCs. Their safety was evaluated in immunodeficient BALB/c nude mice. Smaller AD-MSCs revealed distinct growth properties, with the cell monolayer rolling up into a large aggregate. These cells had longer telomeres (18,121.43 base pairs [bp]) than the total population (15,870.44 bp) and formed teratoma-like structures with skin-like morphology (including hair). In conclusion, AD-MSC size reliably isolates cells with longer telomeres and potential.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-06-06T03:19:03.21Z","creation":"2026-06-06T03:10:26.22Z"},"accession":"S-EPMC12823908","cross_references":{"pubmed":["41582951"],"doi":["10.3389/fbioe.2025.1687461"]}}