{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["17(1)"],"submitter":["Zhou H"],"pubmed_abstract":["The role of RNA N<sup>6</sup>-methyladenoine (m<sup>6</sup>A) eraser AlkB homologue 5 (ALKBH5) in colorectal cancer (CRC) stem cells (CSCs) is unclear. Here, we find that ALKBH5 expression positively correlates with CSC markers in CRC patients. ALKBH5 induces self-renewal and stemness markers in colorectal CSCs and patient-derived organoids (PDOs). Colon-stem cell specific Alkbh5 knockin accelerates carcinogen-induced CRC, while tumorigenesis is attenuated in colon-stem cell specific Alkbh5 knockout mice. Integrated RNA-seq, MeRIP-seq and Ribo-seq reveal FAM84A as an ALKBH5 target. ALKBH5 demethylates m<sup>6</sup>A-modified FAM84A mRNA, causing mRNA decay and reduced expression. Mechanistically, we show that FAM84A represses CSCs by interacting with β-catenin and promoting β-catenin ubiqu"],"journal":["Nature communications"],"pagination":["803"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12824147"],"repository":["biostudies-literature"],"pubmed_title":["Targeting of the m&lt;sup&gt;6&lt;/sup&gt;A eraser ALKBH5 suppresses stemness and chemoresistance of colorectal cancer."],"pmcid":["PMC12824147"],"pubmed_authors":["Cheung H","Kang W","Yuan K","Wong CC","Chen H","Yu J","Su H","Liu W","Liang C","Ding Y","Wei Q","Li T","Wang S","Cheung AHK","Luo W","Chen S","Zhou H","Wu Y"],"additional_accession":[]},"is_claimable":false,"name":"Targeting of the m&lt;sup&gt;6&lt;/sup&gt;A eraser ALKBH5 suppresses stemness and chemoresistance of colorectal cancer.","description":"The role of RNA N<sup>6</sup>-methyladenoine (m<sup>6</sup>A) eraser AlkB homologue 5 (ALKBH5) in colorectal cancer (CRC) stem cells (CSCs) is unclear. Here, we find that ALKBH5 expression positively correlates with CSC markers in CRC patients. ALKBH5 induces self-renewal and stemness markers in colorectal CSCs and patient-derived organoids (PDOs). Colon-stem cell specific Alkbh5 knockin accelerates carcinogen-induced CRC, while tumorigenesis is attenuated in colon-stem cell specific Alkbh5 knockout mice. Integrated RNA-seq, MeRIP-seq and Ribo-seq reveal FAM84A as an ALKBH5 target. ALKBH5 demethylates m<sup>6</sup>A-modified FAM84A mRNA, causing mRNA decay and reduced expression. Mechanistically, we show that FAM84A represses CSCs by interacting with β-catenin and promoting β-catenin ubiqu","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-06T22:00:16.482Z","creation":"2026-06-05T03:11:56.825Z"},"accession":"S-EPMC12824147","cross_references":{"pubmed":["41390849"],"doi":["10.1038/s41467-025-67502-0"]}}