biostudies-literatureNath AMats Paulssons StiftelseSten K Johnsons stiftelseVetenskapsr?det1252-1261https://www.ebi.ac.uk/biostudies/studies/S-EPMC12824985biostudies-literatureUnknown98(2)We present an integrated acoustofluidic plasmapheresis system designed for ultralow blood volume applications, such as neonatal care, enabling in-line sampling and plasma separation from whole blood. The system combines a two-stage acoustophoresis chip with microperistaltic pumps and PDMS-based flow pulsation dampeners to ensure continuous, stable operation. The input whole blood is acoustically separated into a cell-free plasma fraction and a returnable cell fraction, enabling closed-loop operation for neonates who have a circulating blood volume as low as 50 mL. The system achieves a plasma generation rate of 27.5 μL/min with ∼100% cell removal, outperforming previous microfluidic plasma separation approaches in terms of purity, throughput, and minimal sample volume. Plasma quality was vAnalytical chemistryAcoustofluidic Plasmapheresis System Designed for Ultralow Blood Volume Applications.PMC128249852019-00795Nilsson LLaurell TQiu WLarsson SMThymann TGram MBaasch TPankratova SLey DLenshof ANath AfalseAcoustofluidic Plasmapheresis System Designed for Ultralow Blood Volume Applications.We present an integrated acoustofluidic plasmapheresis system designed for ultralow blood volume applications, such as neonatal care, enabling in-line sampling and plasma separation from whole blood. The system combines a two-stage acoustophoresis chip with microperistaltic pumps and PDMS-based flow pulsation dampeners to ensure continuous, stable operation. The input whole blood is acoustically separated into a cell-free plasma fraction and a returnable cell fraction, enabling closed-loop operation for neonates who have a circulating blood volume as low as 50 mL. The system achieves a plasma generation rate of 27.5 μL/min with ∼100% cell removal, outperforming previous microfluidic plasma separation approaches in terms of purity, throughput, and minimal sample volume. Plasma quality was v2026-01-01T00:00:00Z2026 Jan2026-06-06T20:10:56.171Z2026-06-04T03:14:18.987ZS-EPMC128249854149399110.1021/acs.analchem.5c04042