biostudies-literaturePatel CNCI NIH HHSNational Institutes of HealthNIGMS NIH HHSNew Jersey Commission on Cancer Research103355https://www.ebi.ac.uk/biostudies/studies/S-EPMC12828330biostudies-literatureUnknown111Current chemotherapy regimens have significantly improved overall survival for children with cancer. However, these treatments are associated with detrimental side effects like chemotherapy-induced cognitive impairment (CICI), or "chemobrain." Measurable deficits in cognitive function persist years after treatment. Specifically, doxorubicin (DOXO), a commonly used chemotherapeutic agent in curative regimens for children with cancer, plays a pivotal role in the development of CICI, even though it doesn't cross the blood-brain barrier (BBB). Using a juvenile rat model, we found that DOXO compromises the BBB integrity. To further address the poorly understood mechanism of DOXO-related CICI, we utilized human cerebral microvascular endothelial cells (hCMEC/D3) to study the changes induced by DNeurotoxicologyThe effects of doxorubicin on blood-brain barrier integrity in hCMEC/D3.PMC12828330R01 CA293210T32 GM135141COCR24PRF009Cole PDPatel CGlytsou CJang MHfalseThe effects of doxorubicin on blood-brain barrier integrity in hCMEC/D3.Current chemotherapy regimens have significantly improved overall survival for children with cancer. However, these treatments are associated with detrimental side effects like chemotherapy-induced cognitive impairment (CICI), or "chemobrain." Measurable deficits in cognitive function persist years after treatment. Specifically, doxorubicin (DOXO), a commonly used chemotherapeutic agent in curative regimens for children with cancer, plays a pivotal role in the development of CICI, even though it doesn't cross the blood-brain barrier (BBB). Using a juvenile rat model, we found that DOXO compromises the BBB integrity. To further address the poorly understood mechanism of DOXO-related CICI, we utilized human cerebral microvascular endothelial cells (hCMEC/D3) to study the changes induced by D2025-01-01T00:00:00Z2025 Dec2026-06-06T22:04:31.763Z2026-06-05T03:12:15.413ZS-EPMC128283304126565210.1016/j.neuro.2025.103355