{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16"],"submitter":["Kafer JM"],"pubmed_abstract":["<h4>Introduction</h4>Soybean agglutinin (SBA) is a major antinutritional factor in soybean seeds, reducing digestibility in monogastric animals. The Le1 gene encodes the primary lectin accumulated in seeds. Genome editing offers a direct strategy to eliminate this factor in elite cultivars.<h4>Methods</h4>Two gRNAs targeting Le1 were inserted into a CRISPR/Cas9 binary vector and used for Agrobacterium tumefaciens-mediated transformation of the soybean cultivar BRS 537. Edited plants were screened by PCR, Sanger sequencing, protein electrophoresis (SDS-PAGE), hemagglutination assays, and segregating generations were tested to identify transgene-free progeny. Agronomic traits were evaluated under field conditions.<h4>Results</h4>Twenty transformation events were generated, with an editing ef"],"journal":["Frontiers in plant science"],"pagination":["1737513"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12829480"],"repository":["biostudies-literature"],"pubmed_title":["CRISPR/Cas9-mediated inactivation of the soybean agglutinin &lt;i&gt;Le1&lt;/i&gt; gene to improve grain quality."],"pmcid":["PMC12829480"],"pubmed_authors":["Kafer JM","Nepomuceno AL","Girotto L","Rech EL","Hoshino RT","Koltun A","Mertz-Henning LM","Marin SRR","Silveira CA"],"additional_accession":[]},"is_claimable":false,"name":"CRISPR/Cas9-mediated inactivation of the soybean agglutinin &lt;i&gt;Le1&lt;/i&gt; gene to improve grain quality.","description":"<h4>Introduction</h4>Soybean agglutinin (SBA) is a major antinutritional factor in soybean seeds, reducing digestibility in monogastric animals. The Le1 gene encodes the primary lectin accumulated in seeds. Genome editing offers a direct strategy to eliminate this factor in elite cultivars.<h4>Methods</h4>Two gRNAs targeting Le1 were inserted into a CRISPR/Cas9 binary vector and used for Agrobacterium tumefaciens-mediated transformation of the soybean cultivar BRS 537. Edited plants were screened by PCR, Sanger sequencing, protein electrophoresis (SDS-PAGE), hemagglutination assays, and segregating generations were tested to identify transgene-free progeny. Agronomic traits were evaluated under field conditions.<h4>Results</h4>Twenty transformation events were generated, with an editing ef","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-06-13T05:15:06.745Z","creation":"2026-06-13T03:08:26.039Z"},"accession":"S-EPMC12829480","cross_references":{"pubmed":["41584671"],"doi":["10.3389/fpls.2025.1737513"]}}