{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Fang L"],"funding":["U.S. Department of Health & Human Services | National Institutes of Health (NIH)","U.S. Department of Health & Human Services | National Institutes of Health","NIGMS NIH HHS"],"pagination":["1296-1305"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12829982"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(9)"],"pubmed_abstract":["The presence of a hydroxyl group at the 2'-position in its ribose makes RNA susceptible to hydrolysis. Stabilization of RNAs for storage, transport and biological application thus remains a serious challenge, particularly for larger RNAs that are not accessible by chemical synthesis. Here we present reversible 2'-OH acylation as a general strategy to preserve RNA of any length or origin. High-yield polyacylation of 2'-hydroxyls ('cloaking') by readily accessible acylimidazole reagents effectively shields RNAs from both thermal and enzymatic degradation. Subsequent treatment with water-soluble nucleophilic reagents removes acylation adducts quantitatively ('uncloaking') and recovers a remarkably broad range of RNA functions, including reverse transcription, translation and gene editing. Fur"],"journal":["Nature chemistry"],"pubmed_title":["Reversible 2'-OH acylation enhances RNA stability."],"pmcid":["PMC12829982"],"funding_grant_id":["GM127295","R35 GM145357","R01 GM127295"],"pubmed_authors":["Jun YW","Xiao L","Kool ET","Fang L","Onishi Y"],"additional_accession":[]},"is_claimable":false,"name":"Reversible 2'-OH acylation enhances RNA stability.","description":"The presence of a hydroxyl group at the 2'-position in its ribose makes RNA susceptible to hydrolysis. Stabilization of RNAs for storage, transport and biological application thus remains a serious challenge, particularly for larger RNAs that are not accessible by chemical synthesis. Here we present reversible 2'-OH acylation as a general strategy to preserve RNA of any length or origin. High-yield polyacylation of 2'-hydroxyls ('cloaking') by readily accessible acylimidazole reagents effectively shields RNAs from both thermal and enzymatic degradation. Subsequent treatment with water-soluble nucleophilic reagents removes acylation adducts quantitatively ('uncloaking') and recovers a remarkably broad range of RNA functions, including reverse transcription, translation and gene editing. Fur","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Sep","modification":"2026-06-13T05:06:34.26Z","creation":"2026-06-13T03:08:42.59Z"},"accession":"S-EPMC12829982","cross_references":{"pubmed":["37365334"],"doi":["10.1038/s41557-023-01246-6"]}}