biostudies-literatureDing MKunshan Traditional Chinese Medicine Science and Technology Development Fund ProjectKunshan Social Development ProjectNanjing University of Chinese Medicine Natural Science Foundation ProjectKey Projects of Jiangsu Provincial ScienceSuzhou Clinical Key Disease Diagnosis and Treatment Project51https://www.ebi.ac.uk/biostudies/studies/S-EPMC12831259biostudies-literatureUnknown21(1)<h4>Background</h4>Non-alcoholic fatty liver disease (NAFLD), often accompanied by insulin resistance, obesity, and hyperlipidemia, is a challenging metabolic disorder to treat. Ge-Lian Qi-Shen Decoction, a traditional Chinese herbal formula, has been clinically used to alleviate symptoms associated with NAFLD, but its underlying mechanisms remain unclear.<h4>Methods</h4>A NAFLD model was established in C57BL/6J mice using a high-fat diet (HFD). The effects of 4-week GQD intervention at different doses on NAFLD-related symptoms were assessed using biochemical analyses, pathological sections, and oral glucose tolerance tests. ELISA and qPCR were employed to investigate the impact of GQD on serum GLP-1 levels and intestinal Gcg gene expression in NAFLD mice. The direct stimulatory effects ofChinese medicineTargeting G-protein-coupled receptors and gut microbiota: Ge-Lian Qi-Shen decoction elevates GLP-1 to combat non-alcoholic fatty liver disease.PMC12831259ZD202425LCZX202229XZR2024193KS2233KZYY2209Peng HGuo MLuo ZZhang ZShan JXiao ZXu RXu CHou XDing MfalseTargeting G-protein-coupled receptors and gut microbiota: Ge-Lian Qi-Shen decoction elevates GLP-1 to combat non-alcoholic fatty liver disease.<h4>Background</h4>Non-alcoholic fatty liver disease (NAFLD), often accompanied by insulin resistance, obesity, and hyperlipidemia, is a challenging metabolic disorder to treat. Ge-Lian Qi-Shen Decoction, a traditional Chinese herbal formula, has been clinically used to alleviate symptoms associated with NAFLD, but its underlying mechanisms remain unclear.<h4>Methods</h4>A NAFLD model was established in C57BL/6J mice using a high-fat diet (HFD). The effects of 4-week GQD intervention at different doses on NAFLD-related symptoms were assessed using biochemical analyses, pathological sections, and oral glucose tolerance tests. ELISA and qPCR were employed to investigate the impact of GQD on serum GLP-1 levels and intestinal Gcg gene expression in NAFLD mice. The direct stimulatory effects of2026-01-01T00:00:00Z2026 Jan2026-06-13T05:17:32.251Z2026-06-13T03:08:38.203ZS-EPMC128312594158082010.1186/s13020-025-01305-9