{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7"],"submitter":["Su YY"],"pubmed_abstract":["<h4>Background</h4>Single-cell RNA sequencing (scRNA-seq) is a powerful tool which can unveil regulatory connections between genes and cells. However, due to the high demand for tissue quality, most scRNA-seq for pancreatic cancer is carried out by surgical specimen. This study (NCT05767697) aims to gain practical experience in carrying out scRNA-seq using endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB).<h4>Materials and methods</h4>With a within-subjects design, two punctures from the same lesion, with a negative pressure of 5 ml (suction group) and without applying suction (non-suction group), were evaluated. Each biopsy sample was separated into three parts: white tissue, red material, and buffer for living cell counting. scRNA-seq was carried out according to the manufacturer"],"journal":["ESMO gastrointestinal oncology"],"pagination":["100113"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12836601"],"repository":["biostudies-literature"],"pubmed_title":["Single-cell RNA sequencing via endoscopic ultrasound-guided fine-needle biopsy for pancreatic tumors uncovered an aggressive subclone with a poor prognosis."],"pmcid":["PMC12836601"],"pubmed_authors":["Chen LT","Huang CJ","Cheng SM","Hsu CW","Liu YS","Yeh CM","Lin MY","Hou YC","Hwang DY","Su YY","Chang WL","Chao YJ","Shan YS","Yu CC"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell RNA sequencing via endoscopic ultrasound-guided fine-needle biopsy for pancreatic tumors uncovered an aggressive subclone with a poor prognosis.","description":"<h4>Background</h4>Single-cell RNA sequencing (scRNA-seq) is a powerful tool which can unveil regulatory connections between genes and cells. However, due to the high demand for tissue quality, most scRNA-seq for pancreatic cancer is carried out by surgical specimen. This study (NCT05767697) aims to gain practical experience in carrying out scRNA-seq using endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB).<h4>Materials and methods</h4>With a within-subjects design, two punctures from the same lesion, with a negative pressure of 5 ml (suction group) and without applying suction (non-suction group), were evaluated. Each biopsy sample was separated into three parts: white tissue, red material, and buffer for living cell counting. scRNA-seq was carried out according to the manufacturer","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Mar","modification":"2026-07-15T12:27:18.709Z","creation":"2026-07-04T03:13:05.395Z"},"accession":"S-EPMC12836601","cross_references":{"pubmed":["41646484"],"doi":["10.1016/j.esmogo.2024.100113"]}}