biostudies-literatureShinohara IThe Robert L. and Mary Ellenberg Chair in Surgery, Stanford University116https://www.ebi.ac.uk/biostudies/studies/S-EPMC12837465biostudies-literatureUnknown13(1)This study establishes a murine model of corticosteroid-associated osteonecrosis of the femoral head (ONFH) using a sustained-release prednisolone pellet and evaluates mitochondrial stress using ¹⁸F-fluorodeoxyglucose positron emission tomography/computed tomography (PET/CT) and changes in key histologic markers of bone over a 6-week period. Sixteen 12-week-old Balb/C mice were divided into two groups: a prednisolone group (PRED) and a control group (SHAM). The PRED group received a subcutaneous 60-day sustained-release pellet containing 2.5 mg of prednisolone, while the SHAM group received placebo pellets. PET/CT imaging was performed at 1, 3, and 6 weeks. Bone mineral density (BMD) measurements, and histomorphological analyses for the number of empty lacunae, osteoblasts, osteBioengineering (Basel, Switzerland)A Novel Murine Model to Study the Early Biological Events of Corticosteroid-Associated Osteonecrosis of the Femoral Head.PMC12837465NAShinohara ISusuki YMurayama MChow SKMorita MCheung PUno TGao QMa CBeinat CGoodman SBIgei TMoses ASfalseA Novel Murine Model to Study the Early Biological Events of Corticosteroid-Associated Osteonecrosis of the Femoral Head.This study establishes a murine model of corticosteroid-associated osteonecrosis of the femoral head (ONFH) using a sustained-release prednisolone pellet and evaluates mitochondrial stress using ¹⁸F-fluorodeoxyglucose positron emission tomography/computed tomography (PET/CT) and changes in key histologic markers of bone over a 6-week period. Sixteen 12-week-old Balb/C mice were divided into two groups: a prednisolone group (PRED) and a control group (SHAM). The PRED group received a subcutaneous 60-day sustained-release pellet containing 2.5 mg of prednisolone, while the SHAM group received placebo pellets. PET/CT imaging was performed at 1, 3, and 6 weeks. Bone mineral density (BMD) measurements, and histomorphological analyses for the number of empty lacunae, osteoblasts, oste2026-01-01T00:00:00Z2026 Jan2026-06-13T03:25:59.675Z2026-06-13T03:12:41.41ZS-EPMC128374654159604710.3390/bioengineering13010116